除草剂丁草胺对蟾蜍脑和肝的毒性及氧化应激效应
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摘要：
以中华大蟾蜍为研究对象,通过评价除草剂丁草胺对大脑和肝脏的毒性作用及与氧化应激的相关性,揭示除草剂的广泛应用对生态系统的潜在危害.将成年蟾蜍随机分组,分别暴露于0,3,15,30 mg/L的丁草胺,并于3,6,9 d后每组分别随机选取16只蟾蜍测定脑和肝脏抗氧化系统指标,用尼氏法染色比较各组蟾蜍端脑海马区形态学变化.结果显示,各试验组均呈脑蛋白质量浓度降低而肝蛋白质量浓度升高的趋势.随丁草胺暴露时间延长,脑内SOD活性先升后降;肝脏SOD活性随丁草胺浓度升高而先升后降;脑内MDA质量浓度和CAT活性的变化与肝脏相同.MDA质量浓度随药物浓度和作用时间的增加而增加,除低浓度组外,肝脏CAT活性均受到抑制.随药物浓度增加,端脑海马区细胞逐渐溶解,空泡化现象加剧,30 mg/L组海马区侧脑室因细胞部分溶解使侧脑室界限模糊,表明丁草胺对蟾蜍大脑和肝脏均表现一定的毒性作用.
Bufo bufo gargarigans was employed to investigate the toxic effects of the herbicide butachlor on brain and liver of toad and its correlation with oxidative stress, and to reveal the potential harm of the wide application of herbicides to the ecosystem. Bufo bufo gargarigans were randomly assigned to butachlor exposure tests at 3, 15, and 30 mg/L, respectively. No drug was used as control group. After 3, 6 and 9 days of drug treatment, 16 toads were randomly selected from each group, respectively. The morphological characteristics of brain hippocampus were compared by Nissl staining. The results showed that the contents of brain protein decreased in each drug group, while the contents of liver protein increased. SOD activities in brain all increased and then decreased following with increasing of treatment time. SOD activities induced in liver increased and then decreased rapidly following with the increasing treatment time. The changes of malondialdehyde content and CAT activity in brain were same as those in liver. MDA content increased with the increasing concentration and time, and CAT activity in liver was inhibited except in low concentration group. With the increasing toxin concentration, the cells in the hippocampus area of the distal brain dissolved gradually, and the phenomenon of cavitation was intensified. In the 30 mg/L group, the boundary of the lateral ventricle of the hippocampus was blurred because of partial dissolution of the cells. The results showed that butachlor exhibited certain toxic effects on brain and liver of toad.
作者：
梁传成 李丽妍 王丽文 邵然
Liang Chuancheng;Li Liyan;Wang Liwen;Shao Ran(Life Science College,Shenyang Normal University,Shenyang 110034,China;Medical School,Huanghe Science and Technology University,Zhengzhou 450063,China)
机构地区：
沈阳师范大学生命科学学院 黄河科技学院医学院
出处：
《betway官方app 学报：自然科学版》 CAS 北大核心 2022年第5期138-142,共5页
Journal of Henan Normal University(Natural Science Edition)
基金：
国家自然科学基金(31970410) 河南省高等学校重点科研项目(21B350001).
关键词：
除草剂 丁草胺 脑和肝脏毒性 氧化应激
herbicides butachlor brain and liver toxicity oxidative stress
分类号：
R96 [医药卫生—药理学]
