高强度间歇训练和中等强度持续训练对高脂膳食雌性小鼠肝脏巨噬细胞浸润的影响

摘要：
为比较高强度间歇训练和中等强度持续训练对高脂膳食雌性小鼠肝脏巨噬细胞浸润的差别.采用3周龄雌性C57BL/6J小鼠分组进行高脂或普通膳食喂养,15周后从高脂膳食组筛选出超重小鼠,随机分为高脂对照组(HFD),中等强度持续训练组(MICT)和高强度间歇训练组(HIIT),继续高脂喂养.其中MICT和HIIT组小鼠进行12周跑台干预,坡度25°,5次/周.运动方案:MICT组采用50%~70%VO2peak的运动强度,持续45 min;HIIT组进行1 min高强(90%~100%VO2peak)+2 min低强(50%~70%VO2peak),每次跑动距离与MICT组相等.与HFD组相比,MICT和HIIT均能降低高脂膳食雌性小鼠肝组织TG含量,减少肝组织内空泡和脂滴,改善肝组织损伤,抑制肝组织内M1型巨噬细胞相关基因和蛋白表达,促进M2型巨噬细胞相关基因和蛋白表达,但HIIT组优于MICT组.运动距离相同情况下,HIIT比MICT更能改善高脂膳食雌性小鼠肝细胞脂肪变性,降低肝组织炎症状态,这可能与肝内巨噬细胞从M1极化为M2型有关.

The purpose of this study was to investigate the effects of high-intensity interval training(HIIT)and moderate-intensity continuous training(MICT)on liver macrophage infiltration in high-fat diet female mice.Female C57BL/6J(3 weeks old)mice were fed with a high-fat diet or ordinary diet.Overweight mice were screened from the high-fat diet group after 15 weeks and randomly allocated into:high-fat control group(HFD),moderate-intensity continuous training group(MICT)and high-intensity interval training group(HIIT),continued to receive high fat feeding.Exercise protocols:mice in MICT and HIIT groups run on the treadmill with a slope of 25°,5 times per week for 12 weeks.MICT adopted 50%-70%VO2peakfor 45 min;HIIT adopted 1 min(90%-100%VO2peak)+2 min(50%-70%VO2peak),the running distance was the same as MICT.Compared with HFD,MICT and HIIT reduced the content of TG,vacuoles and lipid droplets in liver tissue of mice with high-fat diet,which lessened injury of hepatic tissue;MICT and HIIT potently repressed mRNA and protein expression of M1 macrophage-related genes,while they potently promoted mRNA and protein expression of M2 macrophage-related genes.Furthermore,we found HIIT group was superior to MICT group.Under the same running distance,HIIT improve hepatocyte steatosis and reduce liver inflammation in high-fat diet female mice better than MICT,which may be related to the polarization from M1 to M2 of intrahepatic macrophages.

作者：
王蕊 何玉秀

Wang Rui;He Yuxiu(College of Physical Education,Henan Normal University,Xinxiang 453007,China;College of Physical Education,Hebei Normal University,Shijiazhuang 050024,China)

机构地区：
betway官方app 体育学院 河北师范大学体育学院

出处：
《betway官方app 学报：自然科学版》 CAS 北大核心  2022年第4期136-143,共8页

Journal of Henan Normal University(Natural Science Edition)

基金：
河南省科技攻关项目(202102310324) betway官方app 博士基金(XJ2021003501)。

关键词：
高强度间歇训练 中等强度持续训练 雌性小鼠 肝脏 巨噬细胞

HIIT MICT female mice liver macrophage

分类号：
R87 [医药卫生—运动医学] 

高强度间歇训练和中等强度持续训练对高脂膳食雌性小鼠肝脏巨噬细胞浸润的影响.pdf