水稻全基因组磷脂酶家族蛋白筛选及其生物信息学分析
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摘要:
利用已鉴定磷脂酶基因同源筛选的方法对水稻全基因组范围的磷脂酶基因进行鉴定与筛选,有助于研究其家族基因的各种功能.通过对水稻全基因组进行分析,筛选鉴定磷脂酶家族成员,并对编码蛋白的跨膜区、等电点、分子量等理化性质以及进化、基因组定位、表达特征进行生物信息分析.结果表明:水稻磷脂酶家族基因分为3个亚组,亚组内基因表现出明显相似的结构及进化特征,且磷脂酶家族基因编码蛋白具有相同的3个保守基序,染色体上的磷脂酶家族基因表现出了明显的不均衡性,磷脂酶家族大部分基因表达量偏低,部分表现出了组织表达偏好性,少部分基因在水稻各个组织或生育阶段表达量比较高,可能同水稻发育相关.
The method of homologous screening of identified phospholipase genes can be used to identify and screen the whole genome-wide phospholipase genes in rice,in favor of further studing the functions of family genes.Through the analysis of rice genome,we screened and identified the members of phospholipase family,and analyzed the physical and chemical properties,such as transmembrane,isoelectric point,molecular weight,evolution,sub-localization and expression characteristics of phospholipase gene encoded protein.The results showed that rice phospholipase family genes were divided into three subgroups,the genes in the subgroup showed obviously similar structure and evolution characteristics,and the proteins encoded by the phospholipase family genes had the same three conserved motifs,and the phospholipase family genes on chromosome showed obvious imbalance,most of OsPLX genes were low expressed,some of them showed tissue expression preference,and a few of them were high expressed in various tissues or growth stages of rice,which may be related to rice development.
作者:
曹梦雨 桑世飞 王亚男 周静 王君怡 张文玲 姬生栋
Cao Mengyu;Sang Shifei;Wang Yanan;Zhou Jing;Wang Junyi;Zhang Wenling;Ji Shengdong(College of Life Sciences,Henan Normal University,Xinxiang 453007,China;Henan Seed Management Station,Zhengzhou 450046,China)
机构地区:
betway官方app 生命科学学院 河南省种子管理站
出处:
《betway官方app 学报：自然科学版》 CAS 北大核心 2022年第3期143-149,共7页
Journal of Henan Normal University(Natural Science Edition)
基金:
国家重点研发计划专项(2018YFD0200200) 河南水稻产业体系项目(Z2012-04-G02) 河南省重大科技专项(171100110300-4).
关键词:
磷脂酶 水稻 生物信息 家族基因
phospholipases rice bioinformatics family genes
分类号:
Q37 [生物学—遗传学]
