基于双十二烷基二甲基溴化铵、琼脂糖修饰电极的中心蛋白金属结合性质研究
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摘要:
应用热解石墨电极(pyrolytic graphite electrode,PG)、双十二烷基二甲基溴化铵(Didodecyldimethylammonium bromide,DDAB)修饰电极,以及应用琼脂糖修饰电极研究了八肋游仆虫N端中心蛋白(N-terminal ciliate Euplotes octocarinatus centrin,N-EoCen)、C端中心蛋白(C-EoCen)与Eu3+结合反应的循环伏安特性.研究发现在琼脂糖修饰电极上出现了配合物Eu3+-N-EoCen一对氧化还原峰,与仅有Eu3+存在时相比,负向移动0.24 V.在DDAB修饰电极上出现了配合物Eu3+-N-EoCen微弱的不可逆还原峰.经过计算N-EoCen,C-EoCen与Eu3+结合反应的结合常数β和结合数n,显示N-EoCen与Eu3+形成1∶2型分子复合物,结合常数β=1.26×107 L·mol-1;C-EoCen与Eu3+结合比为1∶2,结合常数β=1.38×109 L·mol-1.
The interaction between N-terminal ciliate Euplotes octocarinatus centrin(N-EoCen),C-terminal EoCen(C-EoCen)and Eu3+was investigated by cyclic voltammetry and pulse voltammetry using a Didodecyldimethylammonium bromide(DDAB)and agarose modified pyrolytic graphite electrode.It was found that a pair of REDOX(oxidation-reduction)peaks of the complex Eu3+-N-EoCen appeared on the agarose modified electrode,which moved in a negative of 0.24 V compared with the presence of Eu3+alone.However,a weak irreversible reduction peak of the complex Eu3+-N-EoCen appeared on the DDAB modified electrode.The binding constantβof Eu3+to N-EoCen(C-EoCen)and the binding number n could be obtained.From the intercept and slope the n=2 andβ=1.26×107 L·mol-1 are deduced for N-EoCen,which indicated that a stable 2∶1 complex of Eu3+-N-EoCen was formed.The binding ratio between C-EoCen and Eu3+is 1∶2,and the binding constantβ=1.38×109 L·mol-1.
作者:
荣志江 孙红艳 杨艳 周焕新 杨斌盛
Rong Zhijiang;Sun Hongyan;Yang Yan;Zhou Huanxin;Yang Binsheng(School of Chemical and Biological Engineering,Taiyuan University of Science and Technology,Taiyuan 030024,China;Institute of Molecular Science,Shanxi University,Taiyuan 030006,China)
机构地区:
太原科技大学化学与生物工程学院 山西大学分子科学研究所
出处:
《betway官方app 学报：自然科学版》 CAS 北大核心 2021年第1期50-56,共7页
基金:
国家自然科学基金(21571117,31401319) 山西省重点研发计划项目(201903D221066) 山西省回国留学人员科研资助项目(2017-083) 太原科技大学博士科研基金(20162018).
关键词:
中心蛋白 循环伏安 DDAB 琼脂糖
centrin cyclic voltammetry DDAB agarose
分类号:
O646 [理学—物理化学]
