微生物絮凝剂对小球藻细胞氧化应激研究

摘要:

小球藻作为能源藻,可以提供产生物柴油,但是小球藻生物质的获取成为能源藻发展的瓶颈.目前研究藻细胞生物质收集的方法很多,但不同收集方法对能源藻细胞的氧化应激研究却很少.分别研究絮凝功能细菌xn-1所产生物絮凝物质和离心法对能源藻——小球藻生物质在收集过程中对藻细胞的氧化应激,以期确定絮凝微生物对小球藻细胞的生物安全性.采用涂布划线法从藻际分离纯化絮凝功能微生物;通过梯度醇沉法获得絮凝物质;分别用絮凝微生物所分泌絮凝物质和离心法收集藻细胞生物质,并对藻细胞内蛋白含量、总糖含量、丙二醛含量(MDA)、过氧化氢酶(CAT)活性、谷胱甘肽(GSH)含量和抗坏血酸(AsA)含量进行测定;以分光光度计测定其吸光度值.经过测定并经过统计学显著性分析,发现离心作用下收集的藻细胞内的MDA,GSH和AsA含量显著高于利用絮凝物质絮凝得到的藻细胞.离心作用相对于絮凝作用造成藻细胞膜发生更高的脂质过氧化,产生大量的活性氧自由基,诱导非酶抗氧化系统响应.因此,离心法收集藻生物质相对于微生物絮凝对藻细胞的伤害更大,絮凝微生物比离心法更适合于收集能源藻生物质.

Chlorella vulgaris,as an energy producing algae,can produce biodiesel,but harvest of C.vulgaris biomass is the bottleneck of the development of algal biodiesel.At present,there are many methods to harvest the algal biomass,but there are few studies on the oxidative stress of algae cells induced by different harvest methods.To investigate the oxidative stress of algae cells induced bioflucculant which produced by flocculation bacterium xn-1 and centrifugation in the process of harvesting algal biomass,in order to ensure the safety of microbial bioflucculant on algal cells.The isolation and purification of flocculation microorganism from phycosphere was performed by spread plate and streak plate methods;the bioflocculant was obtained by gradient ethanol precipitation method;the protein content,total sugar content,the content of MDA,catalase（CAT）activity,glutathione（GSH）and the content of ascorbic acid（AsA）content in algae cell were determined by spectrophotometer.Through the measurement and statistical analysis,it was found that the content of MDA,GSH and AsA in the algae cells collected by centrifugation was significantly higher than that obtained by bioflocculant.Compared with the bioflocculant,the centrifugation caused higher lipid peroxidation on the algal cell membrane,and resulted in a great deal of reactive oxygen species,which induced the non-enzymatic antioxidant system response.Therefore,harvest of algae biomass by centrifugation is more harmful to the algal cells than the flocculation microorganisms,and the flocculating microorganism is more suitable than centrifugation to harvest algal biomass.

作者:

李祎 许艳婷 宋瑞雪 田聪琦 王海磊 郑天凌

Li Yi;Xu Yanting;Song Ruixue;Tian Congqi;Wang Hailei;Zheng Tianling(College of Life Sciences,Henan Normal University,Xinxiang 453007,China;School of Life Sciences,Xiamen University,Xiamen 361005,China)

机构地区:

betway官方app 生命科学学院 厦门大学生命科学学院

出处:

《betway官方app 学报：自然科学版》 CAS 北大核心 2018年第4期81-88,共8页

基金:

国家自然科学基金(51008119 41576109) 河南省高等学校重点科研项目计划(17A610002)

关键词:

小球藻 生物质收集 絮凝微生物 离心法 氧化应激

Chlorella vulgaris harvest of biomass flocculating microorganism centrifugation oxidative stress

分类号:

Q939.9 [生物学—微生物学]

微生物絮凝剂对小球藻细胞氧化应激研究.pdf