青霉素钠与氯化钠对N-乙酰神经氨酸发酵的影响
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摘要：
重组枯草芽孢杆菌生产N-乙酰神经氨酸的过程中,通过加入表面活性剂和抗生素等方法来改善细胞通透性,以减少产物和副产物所带来的反馈抑制,从而促进细胞生长和产物的合成.对比研究了吐温20、吐温60、吐温80及青霉素钠对重组枯草芽孢杆菌摇瓶和5L发酵罐发酵的影响,确定青霉素钠具有明显增强该菌种发酵产N-乙酰神经氨酸的效果.通过对青霉素钠的添加时间和添加量进行了优化,确定在10h和34h均添加200mg·L-1青霉素钠最优,使N-乙酰神经氨酸的产量达到7.17g·L-1,相比对照提高了38.42%.在利用青霉素钠提高目标产物的基础上,又通过在发酵培养基成分中添加0.75%氯化钠来改变细胞渗透压,从而增强细胞通透性.实验证明,N-乙酰神经氨酸的产量进一步增加,最终达到7.96g·L-1.
The present study aimed to enhance the production of N-Acetylneuraminic Acid(Neu5Ac)produced by recombinant Bacillus subtilis.Some methods were studied in the fermentation process,such as adding surfactants and antibiotics,which may allow to weaken the feedback inhibition caused by products and by-products.And the effects of different Tween and penicillin sodium on the fermentation of recombinant Bacillus subtilis in the shake flasks and 5-L fermenter were investigated.As a result,it was confirmed that penicillin sodium could significantly enhance the fermentation yield of N-Acetylneuraminic Acid.Moreover,the optimal time and dosage of penicillin sodium were determined through a series of experimental optimization.The yield of N-Acetylneuraminic Acid reached to 7.17 g·L-1,increased by 38.42%under optimal condition in which 200 mg·L-1 sodium penicillin was added into the medium at 10 h and 34 h,respectively.Based on the action of penicillin sodium,the cell osmotic pressure was adjusted by adding 0.75%sodium chloride into the medium components to enhance cell permeability.The results showed that the production of N-Acetylneuraminic Acid increased further,and eventually the yield of 7.96 g·L-1 was achieved.
作者：
高兰兰 吴金勇 袁丽霞 陈祥松 朱微微 孙立洁 姚建铭
Gao Lanlan;Wu Jinyong;Yuan Lixia;Chen Xiangsong;Zhu Weiwei;Sun Lijie;Yao Jianming(Institute of Plasma Physics,Chinese Academy of Sciences,Hefei 230031,China;University of Science and Technology of China,Hefei 230026,China)
机构地区：
中国科学院合肥物质科学研究院等离子体物理研究所 中国科学技术大学
出处《betway官方app 学报：自然科学版》 CAS 北大核心 2018年第3期74-79,124,共7页
基金：
国家自然科学基金青年项目(21506235) 安徽省自然科学基金面上项目(1508085MB38)
关键词：
枯草芽孢杆菌 N-乙酰神经氨酸 青霉素钠 氯化钠
Bacillus subtilis N-Acetylneuraminic acid penicillin sodium sodium chloride
分类号：
Q939.9 [生物学—微生物学]
