3种水中头孢曲松钠检测方法的对比

摘要：

分别对褪色分光光度法、紫外分光光度法和高效液相色谱法3种头孢曲松钠的检测方法进行了实验对比,实验得到3种方法的检出限分别是0.13、0.028和0.004 7mg·L-1,回收率分别达到78%~81%、92%~96%和99%~101%.实验对高效液相色谱法色谱条件进行了优化,采用Waters~XBridge C18(150 mm×4.6 mm,5μm)色谱柱为固定相,柱温30℃,流动相为甲醇和0.02 mol·L-1三乙胺水溶液(体积比为27∶73),检测波长254nm.实验结果表明,精度要求较高的情况下可以选用高效液相色谱法,在检出限不低于0.03mg·L-1的情况下,紫外分光光度法由于所需仪器价格低廉,不失为一种便捷的方法,尤其在厂矿企业具有巨大的应用价值.

Three detection methods of ceftriaxone sodium in water,including the fading spectrophotometry,the ultraviolet spectrophotometry,and the high performance liquid chromatography were developed.The detection limits and the recoveries of the three methods were0.13,0.028and0.0047mg·L-1and78%-81%,92%-96%and99%-101%,respectively.The chromatographic conditions were optimized as follows.Mobile phase:methanol and0.02mol·L-1triethylamine(the volume ratio is27∶73);column:Waters XBridge C18(150mm×4.6mm,5μm);column temperature:30°C;detection wavelength:254nm.By comparison,the method of high performance liquid chromatography has higher accuracy requirements with the detection limit of not less than0.03mg·L-1case;the method of UV spectrophotometry is easy to apply because of the simple equipment required,especially in water from the factories and mines enterprises.

作者：

崔延瑞 张璐璐 程瑶 吴青 孙剑辉

Cui Yanrui;Zhang Lulu;Cheng Yao;Wu Qing;Sun Jianhui(School of Environment Henan Normal University, Xinxiang 453007, China;Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education,Xinxiang 453007, China;Henan Key Laboratory of Environmental Pollution Control, Xinxiang 453007, China)

机构地区：

betway官方app 环境学院黄淮水环境与污染防治教育部重点实验室河南省环境污染控制重点实验室

出处：

《betway官方app 学报：自然科学版》 CAS 北大核心 2018年第1期84-88,共5页

基金：

河南省高等学校重点科研项目(17A610009) 河南省科技厅重点科技攻关项目(172102310698) 新乡市科技攻关计划项目(CXGG16008).

关键词：

头孢曲松钠 检测方法 检出限 回收率 色谱条件

ceftriaxone sodium detection method detection limit recovery rate chromatographic conditions

分类号：

X502 [环境科学与工程—环境工程]

3种水中头孢曲松钠检测方法的对比.pdf