角毛壳菌丝切蛋白基因的克隆和特性分析

摘要:

在构建的角毛壳菌(Chaetomium cupreum)菌丝体cDNA文库中获得丝切蛋白(Cofilin)的ESTs序列片段,为研究角毛壳菌的生物特性,提高其生物防治效果,以角毛壳菌总RNA反转录产物为模板进行PCR扩增,成功获得了Cofilin基因cDNA序列.生物信息学分析结果表明,该基因全长468bp,编码155个氨基酸,编码蛋白理论分子量17kD,等电点是4.99,α-螺旋和无规则卷曲是其主要的二级结构,为亲水性蛋白质.该蛋白有6个位点发生Ser磷酸化,4个位点发生Tyr磷酸化,有4个保守区,ClustalX分析说明该蛋白与柄孢霉(Podospora anserina)和球毛壳菌(C.g lobosum)亲缘关系较近.

Cof ilin gene was obtained from mycelia cDNA library of Chaetomium cupreum. Full length of Cofilin gene was acquired by PCR amplification with reverse RNA template in order to study the biological character and enhance the biocon-trol effect. The bioinformatics results showed that the full-length of Cofilin gene was 468 bp length and encoded 155 amino acids. The protein molecular weight was 17 kD and its theoretical isoelectric point was 4. 99. Cofilin was hydrophilic and its main secondary structure elements of cofilin were alpha helix and random coil. Cofilin protein included 4 conservative regions and 6 Ser and 4 Tyr phosphorylation site. ClustalX analysis revealed that the amino acid sequence shared high similarity with P. anserina and C. g lobosum.

作者:

李黎 张海燕

机构地区:

河南大学生命科学学院

出处:

《betway官方app 学报：自然科学版》 CAS 北大核心 2017年第3期53-57,共5页

基金:

国家自然科学基金(31400108) 河南大学科研项目(xxjc20140009)

关键词:

角毛壳菌 丝切蛋白 基因克隆 生物信息学分析

C. cupreum, Cofilin-, gene cloning bioinformatics analysis

分类号:

Q785 [生物学—分子生物学]

角毛壳菌丝切蛋白基因的克隆和特性分析.pdf