ACE基因多态性与中老年女性高血压患者急性运动降压效果的关联研究

浏览次数： 12

分享到:

摘要：

目的:探讨血管紧张素转化酶(angiotensin converting enzyme,ACE)基因多态性与女性高血压患者不同强度急性运动后血压和唾液一氧化氮(nitric oxide,NO)含量变化的关系,为高血压患者个性化运动处方提供依据.方法:69名中老年女性高血压患者利用聚合酶链式反应测定ACE基因插入/缺失(insertion/deletionI/D)多态并分为I等位基因型组(II+ID)和DD基因型组.所有受试者分别进行3次实验(每次间隔48h):递增负荷力竭运动实验(incremental exhausted testIET)、中等强度有氧运动实验(moderate aerobic exercise testMAET)和安静对照实验(rest control testRCT).每次实验前后分别测定受试者血压水平和唾液NO含量.结果:3种基因型分布频率(II:24.6%,ID:44.9%,DD:30.4%),符合哈迪温伯格遗传平衡定律(P>0.05).II+ID组IET和MAET后SBP和MAP较实验前降低(P<0.05),RCT后收缩压(systolic blood pressure,SBP)、舒张压(diastolic blood pressure,DBP)和平均动脉压(mean arterial pressure,MAP)较实验前升高(P<0.05);IET后SBP和MAP变化量低于MAET和RCT组(P<0.05),MAET后SBP和MAP变化量低于RCT组(P<0.05);DD组IET和MAET后SBP,DBP和MAP较实验均无显著性差异(P>0.05),RCT后DBP和MAP较实验前升高(P<0.05).II+ID组IET后唾液NO含量较实验前升高(P<0.05),其变化量高于MAET和RCT(P<0.05);DD组不同强度运动后唾液NO含量均无显著性变化(P>0.05).结论:中老年女性高血压患者ACE I等位基因(而非DD基因型)携带者急性运动后SBP和MAP下降、NO升高,且与运动强度正相关.因此ACE基因I/D多态性可影响运动的降压效果与NO释放量.

Objective： To explore the association of angiotensin converting enzyme （ACE） gene polymorphism and post- exercise blood pressure and salivary nitric oxide （NO） responses to acute exercise of different intensity in middle-to-aged female hypertensive patients in order to provide evidence for individual exercise prescription for hypertensive patients. Methods： Sixty- nine middle-to-aged female hypertensive patients were divided into I allelotype group （II-kID） and DD genotype group according to ACE gene insertion/deletion （I/D） polymorphism by polymerase chain reaction. All participants participated three tests （48 h interval in every test）： an incremental exhausted test （IET）, a moderate aerobic exercise test （MAET） and a rest control test （RCT）. Blood pressure level and saliva NO content were determined before and after each trial. Results： The genotype fre- quencies （II： 24.6%, ID： 44. 9%, DD： 30.4%） were coincident with Hardy-Weinberg equilibrium （P〈0.05）. In II/ID group, SBP and MAP reduced after IET and MAET （P〈0.05）, and SBP, DBP and MAP raised （P〈0.05） after RCT com- pared with pre-test （P〈0.05） ; change amplitude of SBP and MAP after IET were lower than MAET and RCT （P〈0.05） while those of SBP and MAP after MAET were lower than RCT （P〈0.05） ; in DD group, SBP, DBP and MAP after MAET had no significantly difference with pre-test （P〈0.05） while DBP and MAP elevated after RCT （P〈0.05）. Saliva NO content increased after IET （P〈0. 05） and change amplitude were higher than MAET and RCT （P〈0.05） in II/ID group; NO con- tent in DD group had no change after different intensity exercise （P〈0.05）. Conclusion： The ACE I allele carriers, but not the DD genotype, presented SBP, MAP reduction and NO upregulation after acute exercise, which was positively related to exer- cise intensity. Therefore, the ACE gene I/D genotype seems exert a role in the NO release and BP response during post-exer- cise recovery in elderly women.

作者：

汤静

机构地区：

河南工程学院体育部

出处：

《betway官方app 学报：自然科学版》 CAS 北大核心 2016年第4期177-183,共7页

基金：

河南省重点科技攻关项目(162102310082)

关键词：

血管紧张素转化酶基因多态高血压运动一氧化氮

angiotensin converting enzyme gene polymorphism hypertension exercise nitric oxide

分类号：