黄河故道湿地可培养细菌多样性及产木聚糖酶菌株筛选

摘要：

研究黄河故道湿地可培养细菌的多样性及其产木聚糖酶的应用潜力.采用稀释平板涂布法从湿地样品中获得119株菌株,形态去重复后对93株进行16SrRNA基因系统发育分析以及利用刚果红染色法进行木聚糖酶活性筛选.结果表明,分离得到的菌株分属于4个门的18个科、21个属,其中优势类群为变形菌门(Proteobacteria)(41株,44%).该地区可培养细菌的Shannon-Wienner多样性指数H′=3.66,Simpson指数D=0.97;Margalef物种丰富度指数dMa=10.59;Shannon物种均匀度指数E=0.94,且样点青龙湖多样性指数高于样点刘寨.93株代表性菌株的木聚糖酶筛选结果显示,11.82%具有木聚糖酶活性,链霉菌和芽孢杆菌在木聚糖酶活性菌株中比例最高.以上结果表明豫北黄河故道湿地蕴含丰富的细菌资源,可为木聚糖酶的研发提供良好的菌种来源.

To investigate the diversity of soil culturable bacteria of the Old Yellow River Course wetland and exploring their xylanase producing activity. Total 119 strains were isolated by using conventional culture-dependent method. After derep lication and removing the similar strains, phylogenetic analysis based on 16S rRNA gene sequences comparisons were further performed. The results showed that the 93 isolates belong to 21 genera of 18 families in 4 phylogenetic groups. The most abun- dant and diverse isolates was the phylum Proteobacteria（41 strains, 44%）. The diversity indices of this region including Shan- non-Wiennerindex（H′）, the Simpson index（D）, the Shannon evenness index（E） and the Margalef index（alMa） were 3.66, 0. 968, 10. 589 and 0. 941 respectively,and the diversity indices of site Qinglong Lake were higher than site Liuzhai. Xylanase screening results showed that the positive rate about 11.82% in 93 strains. The abundant genus were Streptomyces and Bacil- lus. This experimental results showed that there were abundant bacterial species diversity and phylogenetic diversity in the Old Yellow River Course wetland. Meanwhile, these strains exhibited stronger activity of xylanase, which could provide the high- quality experimental for the research and development of functional compounds.

作者：

孟晓林 黄建蓉 明红 郭倩倩 聂国兴

机构地区：

betway官方app 水产学院 新乡医学院生命科学技术学院合成生物学研究所

出处：

《betway官方app 学报：自然科学版》 CAS 北大核心 2016年第3期133-139,153,共8页

基金：

河南省高校科技创新团队支持计划(14IRTSTHN013) 河南省科技创新杰出青年支持计划(154100510009) 河南省教育厅科学技术研究重点项目(14A240001)

关键词：

黄河故道湿地 可培养细菌 多样性 木聚糖酶

Old Yellow River Course wetland culturabl＇e bacteria phylogenetic analysis biodiversity xylanase

分类号：

Q938.2 [生物学—微生物学]

黄河故道湿地可培养细菌多样性及产木聚糖酶菌株筛选.pdf