河南太行山脉3产地淡水三角涡虫染色体数目及核型多样性分析

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摘要：

利用空气干燥法对采自河南境内太行山脉十字岭、张沟、八里沟3产地淡水三角涡虫(Dugesiasp)的染色体和核型进行了研究.结果表明:十字岭淡水三角涡虫(Dugesia sp)体细胞中染色体数目以24条为主(2n=3x=24=24m),占84.56%,少数为16条(2n=2x=16=16m),占8.98%,染色体基数为8,为二倍体和三倍体的混合倍体;张沟淡水三角涡虫(Dugesiasp)体细胞中染色体数目以25条为主(2n=3x+1=24+1SB=21m+3sm+1SB),占80.84%,含有一条小B染色体,少数为24条(2n=3x=24=21m+3sm),占15.99%,极少数为32条,占3.17%,染色体基数为8,为三倍体、四倍体和三倍性非整倍体的混合倍体;八里沟淡水三角涡虫(Dugesiasp)的体细胞中染色体数目以24条(2n=3x=24=24m)和32条(2n=4x=32=32m)为主,分别占38.21%和39.77%,染色体基数为8,为三倍体和四倍体的混合倍体,该产地部分三倍体个体体细胞中染色体结构发生变异,个别染色体末端有随体,其核型呈现多型性.

The chromosomes and karyotype of the freshwater planarians, Dugesia sp, collected from Shiziling, Zhang- gou, Baligou in Taihang mountains, Henan province, were studied by air drying method. The results showed.. （1） The karyo- type of planarians collected from Shiziling belong to mixoploids, consisting of the triploid 24 （2n= 3x= 24=24m） and diploid 16 （2n= 2x= 16= 16m）, the percentage of triploid and diploid cells is 84.56 % and 8.98%, respectively. （2） The karyotype of planarians collected from Zhanggou have the triploid aneuploidy chromosome number of 25 with the karyotype formula of 2n= 3x＋ 1 = 24 ＋ 1SB= 2 lm＋ 3sm＋ 1SB, the triploid that of 24 with one of 2n = 3x = 24 = 2 1m＋ 3sm and tetraploid 2n = 4x = 32. The percentage of triploid aneuploidy, triploid and tetraploid cells is 80.84%, 15.99% and 3.17%, respectively. （3） The kar- yotype of planarians collected from Baligou is mixoploid composed of triploid and tetraploid, the karyotype formula is 2n= 3x= 24= 24m and 2n= 4x = 32 = 32m and the individual chromosomes has satellite in triploid and its karyotype showed polymor- phism, the percentage of triploid and tetraploid cells is 38.21 %and 39. 77%, respectively.

作者：

程方方董自梅李小艳陈广文刘德增

机构地区：

betway官方app 生命科学学院

出处：

《betway官方app 学报：自然科学版》 CAS 北大核心 2016年第3期104-109,114,共7页

基金：

国家自然科学基金(31570376 31471965 31170357) 河南省基础与前沿技术研究基金(142300410160)

关键词：

淡水三角涡虫太行山脉核型染色体

dugesia Taihang mountains karyotype chromosome

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