牛至精油对嗜热四膜虫基因表达的影响
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摘要:
牛至精油(Oregano Essential Oil,OEO)具有抗菌、杀菌、抑制寄生虫生长等功效.为了解牛至精油影响细胞生长的分子调控过程,对牛至精油作用下0 h和24 h的嗜热四膜虫的基因表达情况进行分析.高通量转录组测序结果表明,对照组即未加入牛至精油24 h与0 h的样本数据,比较分析后鉴定到了6297个差异表达基因(包括下调4232个、上调2065个),而实验组即加入牛至精油24 h与0 h的样本数据,比较分析后鉴定到7596个差异表达基因(包括下调6254个、上调1342个).通过与对照组差异表达基因的比较发现,实验组特异上调和下调差异表达基因分别有257个和2329个.GO功能富集发现,特异上调差异表达基因无功能富集,特异下调差异表达基因功能主要富集在RNA加工、蛋白质分解代谢、细胞定位和信号转导等过程.KEGG通路分析发现,特异下调差异表达基因主要涉及细胞自噬及细胞周期等通路.而这些过程或通路上基因表达水平的下调可能会抑制细胞的生长.对通路中7个下调的差异表达基因和3个无明显差异表达变化的基因进行实时荧光定量PCR检测,实验结果和转录组测序数据结果的相关性高.根据相关基因和通路的分析,绘制了牛至精油作用下影响嗜热四膜虫细胞生长的可能模型,为探讨分子调控机理提供线索.
Oregano Essential Oil(OEO)has the functions of antibacterial,bactericidal and inhibition of the parasite cells.In order to understand the molecular regulation mechanism of cells under OEO,the gene expression of Tetrahymena thermophila at 0 h and 24 h was analyzed in this study.Based on the anaysis of high-throughput transcriptome sequencing(RNA-seq)6297 differentially expressed genes(DEGs)were identified in the control group in which there was no OEO at 0 h and 24 h,including 4232 downregulated DEGs and 2065 upregulated DEGs.And,7596 DEGs were identified in the experimental group in which OEO was added at 0 h and 24 h,including 6254 downregulated DEGs and 1342 upregulated DEGs.Compared to the control group,there were 257 specific upregulated DEGs and 2329 specific downregulated DEGs in the experimental group.GO enrichment analysis showed that there was no function enriched among the specific upregulated DEGs and the process of RNA processing,protein catabolic process,cell localization and signal transduction were enriched among specific downregulated DEGs.According to KEGG analysis,downregulated DEGs were involved in autophagy and cell cycle pathway under OEO.The downregulated DEGs in these processes and pathways may inhibit cell growth.7 downregulated DEGs and 3 unchanged genes in pathways were chosen to validate the expression differences by quantitative real time polymerase chain reaction(RT-qPCR).RT-qPCR and RNA-seq were highly correlated to each other.Based on the analysis of relevant genes and pathways,the possible model of T.thermophila cells growth under OEO was analyzed.These provide clues to explore the molecular regulation mechanism under OEO.
作者:
李文秋 张晶 罗帅 胡澈 陈瑛 缪炜
Li Wenqiu;Zhang Jing;Luo Shuai;Hu Che;Chen Ying;Miao Wei(College of Life Science and Technology,Harbin Normal University,Harbin 150025,China;Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China)
机构地区:
哈尔滨师范大学生命科学与技术学院 中国科学院水生生物多样性与保护重点实验室
出处:
《betway官方app 学报：自然科学版》 CAS 北大核心 2023年第1期121-129,I0006,共10页
Journal of Henan Normal University(Natural Science Edition)
基金:
国家自然科学基金(21976208) 武汉市应用基础前沿项目(2019020701011483).
关键词:
牛至精油 嗜热四膜虫 转录组 差异表达基因
oregano essential oil Tetrahymena thermophila transcriptome differentially expressed genes
分类号:
Q75 [生物学—分子生物学]
