胀果甘草细胞悬浮培养体系的非结构动力学分析

摘要:

在建立了稳定的胀果甘草细胞悬浮培养体系的基础上,分析了体系中细胞生长、基质消耗及产物合成的动态变化,利用Excel 2013和Origin Pro 8.0软件处理实验数据,拟合出模型参数,建立了相应的非结构动力学模型.模型模拟值与试验值的拟合度良好,相关系数分别为0.968 46、0.964 17、0.975 64,表明建立的动力学模型能很好地预测甘草细胞的悬浮培养过程,对指导反应器放大培养甘草细胞具有重要意义,同时为植物细胞生物反应器的设计提供理论依据.

Based on the establishment of a stable Glycyrrhiza inflata cell suspension culture system, dynamic changes a- bout cell growth, substrate consumption and product synthesis in the system were analyzed. Using Excel 2013 and Origin Pro 8.0 software to process experimental data, model parameters, corresponding non-structural kinetic models were established. Analog value and experimental values were very consistent, the correlation coefficients were 0. 968 46, 0. 964 17, 0. 975 64 re- spectively. This indicates the dynamic models can well predict suspension culture process of Glycyrrhiza inflata cell. This has important significance to guide the cell suspension culture in the reactor, and to provide a theoretical basis for the bioreactors design.

作者:

孟婷婷 郭慧 郑春丽 李雅丽

机构地区:

内蒙古科技大学数理与生物工程学院

出处:

《betway官方app 学报：自然科学版》 CAS 北大核心 2015年第5期122-126,172,共6页

基金:

国家自然科学基金(31460064) 内蒙古自然科学科金(2013MS0512) 内蒙古自治区青年科技英才计划项目(NJYT-15-A08) 内蒙古自治区高等学校科学研究项目(NJZY13152)

关键词:

甘草细胞 悬浮系 动力学模型 生物反应器

licorice cells suspensions kinetics modeling bioreactors

分类号:

Q813.1 [生物学—生物工程]

胀果甘草细胞悬浮培养体系的非结构动力学分析.pdf