沿黄稻区土壤菌群结构与生态功能研究

摘要：

为了解沿黄稻区不同基质土壤的微生物组成,采集了河滩砂质土壤(sand soil,SS)和毗邻稻田土壤(paddy soil,PS).利用16S rRNA基因高通量测序技术揭示沿黄稻区土壤菌群的组成及其潜在的生态功能.Alpha多样性分析显示两种土壤的菌群丰富度和多样性指数无显著性差异.进一步的物种组成分析,发现unclassified_f_Micrococcaceae、鞘氨醇单胞菌属(Sphingomonas)、norank_f_noorank_o_Vicinamibacterales、norank_f_Vicinamibacterales在SS和PS中广布,与土壤基质类型无关丰度,但芽孢杆菌属(Bacilllus)、硫杆菌属(Thiobacillus)在PS中的相对丰度显著高于SS.基于FAPROTAX数据库的功能热图分析显示PS(尤其是P2_1、P3_1)中芳香烃降解功能序列的相对丰度高于SS,结合菌群相对丰度结果推测类诺卡氏菌属(Nocardioides)、unclassified_f_Nocardioidaceae是该差异产生的关键.SS中光养微生物丰度显著高于PS,尤以卟啉杆菌(Porphyrobacter)、unclassified_f_Rhodobacteraceae、红细菌(Rhodobacter)为主的不产氧光合细菌的丰度较高,其中红细菌科(Rhodobacteraceae)是参与SS生物膜形成的重要微生物.研究表明土壤类型对沿黄稻区菌群组成有一定的影响,并驱动了菌群生态功能分异的形成.

In the present study,samples of riverside sand soil(SS)and adjacent paddy soil(PS)were collected to know the microbial composition of different substrate soils in the rice zone along the Yellow River.The high-throughput sequencing of 16S rRNA gene was used to uncover the soil microbial compositions and to explain microbes'potential eco-function.Alpha diversity analysis showed no significant difference in microbial richness and diversity index between the two soils.Further species analysis showed the ubiquitous distribution of unclassified_f_Micrococcaceae,Sphingomonas,norank_f_noorank_o_Vicinamibacterales and norank_f_Vicinamibacterales,whose abundances were independent of soil types.However,the relative abundances of Bacilllus and Thiobacillus were significantly higher in PS than that in SS.The function heatmap analysis based on FAPROTAX database showed that aromatic compound degrading bacteria in PS(especially P2_1 and P3_1)was higher than that in SS.Combined with the results of bacterial relative abundance,Nocardioides and unclassified_f_Nocardioidaceae might be the key genera contributing to aromatic compound degradation.The phototrophic microbes(PMs)in SS were more abundant than that in PS,which were dominant anoxygenic PMs like Porphyrobacter,unclassified_f_Rhodobacteraceae and Rhodobacter.Moreover,Rhodobacteraceae was likely to participate in biofilm formation in SS.Overall,soil microbial compositions along the Yellow River were to some extent impacted by the soil type,driving the differences of their eco-functions.

作者：

田莹莹 郭琦 陈星儒 孙向辉 赵京

Tian Yingying;Guo Qi;Chen Xingru;Sun Xianghui;Zhao Jing(Department of Material Science and Engineering,Henan Institute of Technology,Xinxiang 453003,China;Xinxiang City Engineering Research Center for Wastewater Treatment Energy Saving and Emission Reduction,Henan Institute of Technology,Xinxiang 453003,China)

机构地区：

河南工学院材料科学与工程学院 河南工学院新乡市水处理节能减排工程技术研究中心

出处：

《betway官方app 学报（自然科学版）》 CAS 北大核心  2024年第3期139-149,I0007,共12页

Journal of Henan Normal University(Natural Science Edition)

基金：

国家自然科学基金(21906041) 河南省科技攻关项目(232102320100,212102310079) 河南省高等学校重点科研项目(21A610003) 河南工学院博士基金项目(9001/KQ1811).

关键词：

壤菌群结构 生态功能 稻田土壤 砂质土壤

soil microbial community structure eco-function paddy soil sand soil

分类号：

S158 [农业科学—土壤学] 

沿黄稻区土壤菌群结构与生态功能研究.pdf