长期低剂量离子辐射诱导小鼠皮层小胶质细胞中 IL-1β表达异常升高的信号转导途径研究
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摘要：
低剂量离子辐射(low-doseionizingradiation,LDIR)的环境暴露对人体健康的影响已成为公共卫生
究领域备受关注的重要问题,但相应的分子机制研究进展缓慢.为探讨 LDIR 对脑功能的潜在危害效应及相关分子
机制,采用8周龄雄性小鼠构建长期间歇性低剂量γ射线(Co60)暴露的实验动物模型,采用免疫荧光、WesternBlot、
荧光定量 PCR(qPCR)等技术,对辐射暴露后小鼠脑组织中的小胶质细胞活化状态、炎症因子表达水平和信号转导
途径中蛋白激酶和转录因子的表达及活化水平进行分析.采用同剂量射线照射小胶质细胞 N9并分析相应指标,用
以模拟小胶质细胞的体内辐射反应状态.结果显示,LDIR 暴露可诱导小鼠大脑皮层中小胶质细胞显著活化,并向
M1型极化.以IL-1β为代表的神经炎症因子表达水平显著升高.N9 细胞经低剂量 γ射线暴露后IL-1β 表达水平上
调,其潜在调节因子c-Jun和 ERKs的活化水平升高.敲低c-Jun的表达水平或抑制 ERKs激酶活性后,IL-1β的诱导
表达反应被显著抑制.实验结果表明,LDIR 暴露可激活小胶质细胞并促进 ERKs活化,进而激活转录因子c-Jun 并
介导IL-1β表达上调反应,这可能在 LDIR诱发小鼠神经炎症反应发生发展中具有重要作用.
As the economic hub ofChina, Shanghaihas witnessed a steady increase in its high-quality developmentover the pastdecade, yetthe transition towards low-carbon sustainability remains a significant challenge. This paper, drawing on public data such as statisticalyearbooks, analyzes the energy evolution trends in Shanghaifrom 2010 to 2020, and employs e- mission factors and input-outputmethods, to identify the carbon footprint associated with energy use in the industrial sector. By establishing three scenariosforfuturedecarbonization forecasts-includinggriddecarbonization, electrification ofenergy use, and hydrogen energy production scenario analysis, this study delves into the potential for emissions reduction through energy use in Shanghai's industrialsector. Itwas found thatcoaland oilconsumption stillaccounts to over60% ofShanghai's totalen- ergy use, remaining the primary sources of energy. Despite significantimprovements in energy efficiency within the industrial sector, there is a heavy reliance on fossilfuel inputs and substantial losses, such as electrical energy loss, with the chemical products industry exhibiting thehighestproportion ofimplicitcarbon footprintin electricity useat30. 9%. Thisunderscoresthe need forongoing efforts in energy conservation, consumption reduction, and green energy substitution. Scenario analysis re- veals thattheemissionsreduction impactofterminalenergyelectrification orhydrogen substitution dependson thedecarboniza- tion ofthe entire upstream industrialchain, with the wind power hydrogen production scenario showing the highest potential for emissions reduction, reaching24. 5%.
作者：
后少俊 , 宋伦
Hou Shaojun, Song Lun.
机构地区：
安徽医科大学基础医学院.；军事医学研究院军事认知与脑科学研究所
引用本文：
长期低剂量离子辐射诱导小鼠皮层小胶质细胞中 IL-1β表达异常升高的信号转导途径研究[J].betway官方app 学报(自然科学版),2024, 52(5) : 134-141.
Signal transduction mecha- nism ofabnormalIL-1βupregulation in themousecortexunderlongtimelow-doseionizing radiation[J] .Journal of Henan NormalUniversity(Natural Science Edition),2024, 52(5):134-141.DOI:10.16366/j.cnki.1000-2367.2024.01.26.0001.
基金：
国家自然科学基金；军队科技重点项目
关键词：
低剂量离子辐射；小胶质细胞；神经炎症;IL-1β
energy-carbon analysis; energy carbon footprint; industrialdecarbonization; scenario analysis; carbon emis- sion reduction
分类号：
Q691. 5
