高效降解邻苯二甲酸酯植物内生菌的抗生素耐药性研究
- 分享到:
摘要：
微生物降解被认为是修复邻苯二甲酸酯(PAEs)最具有前景的方法之一。然而，从环境中分离的PAEs高效降解菌大部分是致病菌或条件致病菌。植物内生菌为非致病菌，分离PAEs高效降解植物内生菌具有重要的现实意义。选取一株Bacillussubtilis N-1为研究对象，该菌株是从受污染的植物内部筛选到的植物内生菌，能以邻苯二甲酸二丁酯(DBP)和邻苯二甲酸二(2-乙基己基)酯(DEHP)两种PAEs为唯一碳源和能源生长。探究了菌株N-1的生长习性，包括其吲哚乙酸的分泌情况和对4种抗生素的耐药性。结果表明，投加色氨酸能 促进菌株N-1分泌吲哚乙酸；0.50mg·L-1庆大霉素、8.00mg·L-1氯霉素、32.00mg·L-1盐酸四环素和32.00mg·L-1利福平为菌株N-1的最小抑菌质量浓度。目前污水中抗生素质量浓度远低于菌株N-1的最小抑菌质量浓度，菌株N-1修复PAEs污染的范围较广泛，具有较好的应用前景。
Microbial degradation is considered as one of the most promising methods for repairing phthalates(PAEs) . However, mosthighly efficient PAEs-degrading bacteria isolated from the environment are pathogenic or conditional patho- gens. Endophytic bacteria are non-pathogenic bacteria. It is of great practical significance to isolate highly efficient PAEs-de- grading endophytic bacteria. Bacillussubtilis N-1 wastheresearch objectin thisstudy, which wasan endophyticbacterium se- lected from a contaminated plant. Strain N-1 could grow using two types of PAEs as sole carbon source and energy source, which were dibutylphthalate(DBP) and di(2-ethylhexyl)phthalate(DEHP) . Its growthhabits were explored, including these- cretion ofindole-3-acetic acid and the resistance to four antibiotics. The results showed that adding tryptophan could promote strain N-1 to secret indole-3-acetic acid. What's more, 0. 50 mg · L- 1 gentamycin, 8. 00 mg · L- 1 chloramphenicol, 32. 00 mg · L- 1 tetracycline and 32. 00 mg · L- 1 rifampicin were the minimum inhibitory mass concentrations for strain N-1. Atpresent, the concentration of antibiotics in wastewater is much lower than the minimum inhibitory mass concentration of strain N-1. Strain N-1has a wide range ofapplication for repairing PAEs pollution and has good prospects forapplication.
作者：
吕辉雄，黄雪晶
Lü Huixiong，Huang Xuejing
机构地区：
华南农业大学资源环境学院；上海交通大学生命科学技术学院；微生物代谢国家重点实验室
引用本文：
吕辉雄，黄雪晶。高效降解邻苯二甲酸酯植物内生菌的抗生素耐药性研究[J].betway官方app 学报(自然科学版) ,2024,52(6) : 20-26. (Lü Huixiong, Huang Xuejing. Study on antibiotic resistance of endophytic bacterium efficiently degrading phthalates[J] .Journalof Henan NormalUniversity(NaturalScience Edition) ,2024,52(6) : 20-26. DOI:10. 16366/j. cnki.1000-2367. 2023. 08. 24. 0003. )
基金：
国家自然科学基金
关键词：
邻苯二甲酸酯；降解；植物内生菌；吲哚乙酸；抗生素耐药性
phthalates; degradation; endophytic bacterium; indole-3-acetic acid; antibiotic resistance
分类号：
Q89
