地黄UDP-鼠李糖:鼠李糖基转移酶基因家族的生物信息学和表达分析
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摘要：
尿苷二磷酸鼠李糖基转移酶能将活性鼠李糖基供体转移到其糖基化受体分子上，合成鼠李糖基化的化合物。它们结构多样，活性广泛，具有药理、维持细胞结构、信号转导和化学防御及调节植物体内激素水平等重要作用。迄今，已从多种植物中分离得到这类酶,但有关其基因家族的结构特性与在植物次生代谢产物毛蕊花糖苷生物合成中的作用研究较少，以地黄尿苷二磷酸鼠李糖基转移酶基因(RgURT)家族的4个成员 RgURTI~RgURT4 为材料,利用生物信息学对该家族的特征进行了分析，比较了 RgURT 在高,低毛蕊花糖苷地黄品种块根中的表达模式.结果表明，其 cDNA 大小为645~1422 bp；编码蛋白质分子量为 24.05~53，24kD,由214~473 个氨基酸残基组成属于糖基转移酶 GT-B型超家族，C端具有保守结构域盒(PSPG 盒)，无信号肽，亚细胞定位在细胞质中，二级结构以无规则卷曲和 “=螺旋为主;RgURT4与RgURTI~RgURT3分别聚类为2个，RgURT4蛋白与其他3个 URT蛋白序列之间的同源性低；RT-GPCR分析表明，RgURT 基因在高毛蕊花糖苷地黄块根中的表达量高于低毛蕊花糖苷地黄块根中的表达量，研究首次揭示了地黄 RURT 家族的特征,初步验证了 RURT 与地黄毛蕊花糖苷合成相关，将为进一步研究毛蕊花糖苷合成提供了重要酶基因。
Rhamnosyltransferases can transfer active rhamnosyl donors to their rhamnosylated receptor molecules to synthesize rhamnosylated compounds with diverse structures and extensive activities. They play important roles in pharmacolo-gy, maintaining cell structure, signal transduction, chemieal delense and regulating hormone levels in plants. So far, they have been isolated from many plants, However, few studies have been conducted on the structural characteristies of their gene fami-lies and their role in the biosynthesis of acteoside in plants. In this study,We bioinformatics technology to analyze the structural charaeteristies of UR'T gene family in Rehmannia glutinosa (RgURTI-RgURT4), and their expression pattems in higher acteoside level variety and lower acteoside level variety were compared using its four members asmaterials.Their secondary structures mainly were random coilide level variety and lowere clustered into two dilferent groups, respectively,and the homology between RgURT4 protein and RgURTl-RgURT3 was low; RT-qPCR analysis showed that the expressions of three RgUR'T genes in the tuberous root of the high acteoside variety were higher than that in the low acteoside variety. This study revealed the structural features of RgUR'T family of Rehmannia glutinosa , and preliminarily verified the correlation be-tween RgURT gene family and acteoside biosynthesis, and will provide candidate genesfor further study of aeteoside biosyn-thesis.
作者：
周延清，李慧敏
Zhou Yanging,Li Huimin
机构地区：
betway官方app 生命科学学院
引用本文：
周延清，李慧敏，地黄 UDP-鼠李糖:鼠李糖基转移酶基因家族的生物信息学和表达分析[1].betway官方app 学报(自然科学版),2024,52(6):128-134.
(Zhou Yanging, li Huimin, Bioinformatics and expression analyses olUDp-rhamnose :rhamnosyltransferase family in Rehmannia glutinosa[)].Joural of Henan Normal University(Natural Science Edition),2024,52(6):128-134.D01:10.16366/j.cnki1000-2367.2022.12.12.0001.)
基金：
国家自然科学基金；河南省重点研发与推广专项；河南省自然科学基金；河南省高校科技创新团队支持计划项目
关键词：
鼠李糖基转移酶；地黄；RgURT家族的特征；PSPG盒
rhamnosyltransferase; Rehmanmia glutinosa ;characteristies of RgURT gene family members;PSPG box
分类号：
Q94
