Effects of heat precondition on m6A modification in rat muscle tissue
- 分享到:
摘要：
为了解大鼠肌肉中m6A修饰在热预适应过程中的变化,并初步探讨m6A在热预适应过程中发挥的作用.选用24只成年雄性SD大鼠,随机分为2组,12只为预热组,12只为对照组.热预处理组是42℃热处理15 min,每天3次,连续5 d,对照组不做任何处理.处理结束后检测关键酶的转录和翻译表达水平,以及总m6A的变化.与对照组相比,热预处理组的甲基转移酶METTL3和METTL14,KIAA1429的mRNA表达水平均有不同程度上升,METTL3最为显著;去甲基化酶FTO和ALKBH5的mRNA水平下降,FTO蛋白表达水平下降极显著;LC-MS检测发现m6A修饰水平升高.在热预适应过程中,大鼠骨骼肌中m6A甲基化修饰增强,去甲基化减弱,总体m6A水平升高,表明m6A信号通路在大鼠肌肉预适应过程中可能发挥正调控作用.
To observe the changes of m6A modification in rat muscles during heat precondition,and to preliminarily explore the role of m6A in heatprecondition,24 adult male SD rats were randomly divided into 2 groups,12 were preheating group and 12 were control group.The preheating group was treated at 42℃for 15 minutes,3 times a day for 5 consecutive days,and the control group were raised under the room temperature.After the treatment,the transcription and translation expression levels of key enzymes and the changes of total m6A were detected.Compared with the control group,the mRNA expression levels of methyltransferases METTL3 and METTL14 and KIAA1429 in the heat precondition group increased to varying degrees,with METTL3 being the most significant.The mRNA expression of demethylases FTO and ALKBH5 decreased,and the FTO protein reduced significantly.LC-MS detection revealed that the m6A modification level increased in total RNA.In the process of heatprecondition,m6A methylation modification in rat skeletal muscle is enhanced,demethylation is weakened,and overall m6A level increases,indicating that m6A signaling pathway may play a positive regulatory role.
作者：
张静婷
Zhang Jingting(College of Physical Education,Henan Normal University,Xinxiang 453007,China)
机构地区：
betway官方app 体育学院
出处：
《betway官方app 学报：自然科学版》 CAS 北大核心 2022年第4期144-149,共6页
Journal of Henan Normal University(Natural Science Edition)
基金：
betway官方app 博士科研启动费支持课题(5101069170120)。
关键词：
热预适应 m6A修饰 甲基化酶 去甲基化酶
heat precondition m6Amodification methylase demethylase
分类号：
G804 [文化科学—运动人体科学]
