Astragalus polysaccharides alleviate lipopolysaccharide-induced oxidative stress and inflammation in IPEC-J2 cells
- 分享到:
摘要：
为探究黄芪多糖对脂多糖诱导IPEC-J2细胞炎症反应的影响.将IPEC-J2细胞分为对照组、LPS处理组、ASP处理组和ASP+LPS处理组,分别检测各组细胞活力、抗氧化指数、相关炎症因子含量和mRNA表达水平.结果表明当10 mg/L LPS作用细胞12 h后,显著降低了细胞活力.当100 mg/L ASP预处理细胞4 h后显著提高了细胞的存活率.与对照组相比,LPS组显著提高了MDA含量并降低了SOD,CAT酶活力,显著上调细胞上清液中IL-6,IL-8和TNF-α的含量以及细胞内三者基因mRNA的表达水平,显著下调了IL-10基因mRNA的表达.而采用ASP预处理4 h后可显著降低细胞内MDA含量及提高SOD和CAT酶活力,且显著下调IL-6,IL-8和TNF-α的含量和细胞内三者基因mRNA的表达及显著上调IL-10基因的表达.试验表明ASP可缓解LPS引起的氧化应激和炎症反应,抑制IL-6,IL-8和TNF-α含量及其基因表达,促进IL-10基因表达,从而发挥抗氧化和抗炎作用.
To investigate the effect of astragalus polysaccharides on lipopolysaccharide induced inflammation of IPEC-J2 cells.IPEC-J2 cells were divided into control group,LPS treatment group,ASP treatment group and ASP+LPS treatment group.The cell viability,antioxidant index,inflammatory factors contents and mRNA expression levels of the four groups were detected,respectively.Results showed that cell viability was significantly decreased after the treatment of 10 mg/L LPS for 12 h,when cells were pretreated with 100 mg/L ASP for 4 h,the cell viability was significantly increased.In addition,compared with the control group,the MDA content increased significantly and the SOD and CAT enzyme activities,and the content of IL-6 increased signifieantly,IL-8 and TNF-α,and un-regulated the mRNA expression of IL-6,IL-8 and TNF-αwhile down-regulated the mRNA expression of IL-10 significantly.However,MDA,IL-6,IL-8 and TNF-αcontents and the mRNA expressions of IL-6,IL-8,TNF-αwere significantly reduced and the SOD and CAT activities together with the mRNA expression of IL-10 were significantly increased after the pretreated of ASP for 4 h.Therefore,ASP could alleviate the oxidative stress and inflammatory response caused by LPS,inhibit the contents of IL-6,IL-8 and TNF-αand expression levels of these genes,and promote the expression of IL-10,thereby exerting antioxidant and anti-inflammatory effects.
作者：
崔海燕 纪龙翔 朱宇晴 李卫国
Cui Haiyan;Ji Longxiang;Zhu Yuqing;Li Weiguo(College of Life Sciences,Henan Normal University,Xinxiang 453007,China)
机构地区：
betway官方app 生命科学学院
出处：
《betway官方app 学报：自然科学版》 CAS 北大核心 2022年第4期101-106,共6页
Journal of Henan Normal University(Natural Science Edition)
基金：
国家自然科学基金(31702349) 河南省科技攻关计划(182102310248)。
关键词：
黄芪多糖 IPEC-J2细胞 脂多糖 氧化应激 炎症
astragalus polysaccharides IPEC-J2 cells lipopolysaccharide oxidative stress inflammation
分类号：
Q955 [生物学—动物学]
