Combination effect of perfluorobutanesulfonate and probiotics on thyroid endocrine system of zebrafish

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摘要：

全氟丁烷磺酸(Perfluorobutanesulfonate,PFBS)是一种新污染物,对鱼类甲状腺内分泌系统具有一定损害效应.益生菌能在一定程度上减轻环境污染物对生物体造成的危害,但其能否调节PFBS的甲状腺内分泌干扰效应尚不清楚.通过将成年斑马鱼暴露于不同质量浓度(0、10和100μg/L)PFBS的养殖水中28 d,投喂添加或不添加益生菌(鼠李糖乳杆菌,Lactobacillus rhamnosus)的饲料,测定亲代下丘脑-垂体-甲状腺轴关键基因,以及亲代大脑、血液和子代胚胎中甲状腺激素水平,以探究PFBS与益生菌共暴露对斑马鱼甲状腺内分泌系统的联合效应.结果显示,在激素水平上,PFBS降低了亲代血液T3水平,而添加益生菌能减轻雄鱼血液T3水平的降低.在基因水平上,100μg/L PFBS与益生菌共暴露促进雄鱼大脑tshβ基因表达以应对低水平的T3,并与相应PFBS单独暴露相比差异显著,表明添加益生菌可以减轻高质量浓度PFBS诱导的雄鱼大脑T3水平的降低;PFBS单独暴露能上调雌鱼大脑trα基因表达,而PFBS与益生菌共暴露则上调trα和trβ基因表达,表明添加益生菌能使机体积极地应对PFBS诱导的雌鱼大脑T3水平降低.研究发现PFBS单独暴露能引起斑马鱼的甲状腺内分泌系统紊乱,而PFBS与益生菌共暴露后,益生菌能在一定程度上通过甲状腺激素水平和基因表达调节PFBS的毒性效应.研究结果可为后续PFBS毒性效应的研究及毒性缓解措施的开发提供重要参考.

Perfluorobutanesulfonate(PFBS),an emerging pollutant,could disturb the thyroid endocrine system of fish,while probiotics can potentially mitigate the harmful effect of environmental pollutants on organisms.However,it remains unknown whether probiotics can modulate the toxicity of PFBS in the thyroid endocrine system of aquatic organisms.In this study,adult zebrafish were exposed to a series of PFBS concentrations(0,10 and 100μg/L)for 28 days,and fed with or without probiotics(Lactobacillus rhamnosus).Genes related to the parental hypothalamic-pituitary-thyroid axis as well as thyroid hormone levels in the parental brain,blood and offspring embryos were investigated to explore the thyroid endocrine disruption effect induced by combined exposure of PFBS and probiotics.The results showed that PFBS decreased the T3 level of parental blood,which was alleviated by probiotics in male fish blood.At the gene level,exposure of 100μg/L PFBS with probiotics promoted the tshβgene expression of male fish brain as stimulated by the decreased T3,which is significantly different from that of the PFBS exposure alone,suggesting that the addition of probiotics could also alleviate the decrease of brain T3 induced by high concentration of PFBS.Exposure to PFBS alone up-regulated the expression of trαgene in female fish brain,while coexposure to PFBS and probiotics up-regulated the expression of trαand trβgenes,implying that the addition of probiotics could react to the T3 decrease induced by PFBS in female fish brain more actively.In conclusion,exposure to PFBS alone could cause thyroid endocrine system disruption in zebrafish,while in the combination of PFBS and probiotics,probiotics could modulate these effects of PFBS through thyroid hormone levels and gene expression.Based on the above findings,this study can provide reference for the follow-up study on the toxic effects of PFBS and toxicity mitigation measures.

作者：

陈联国唐丽珠白雅琛

Chen Lianguo;Tang Lizhu;Bai Yachen(State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China)

机构地区：

中国科学院淡水生态与生物技术国家重点实验室

出处：

《betway官方app 学报：自然科学版》 CAS 北大核心 2022年第1期1-11,共11页

Journal of Henan Normal University(Natural Science Edition)

基金：

国家自然科学基金(22006159) 淡水生态与生物技术国家重点实验室开放课题基金(2019000153).

关键词：

全氟丁烷磺酸益生菌甲状腺内分泌基因转录激素

perfluorobutanesulfonate probiotics thyroid endocrine gene transcription hormone

分类号：

Q89 [生物学]