Research progress of paper-based detection methods

摘要：

随着现代检测技术的简约化、快速化、低成本的发展趋势,基于纸的各类检测方法应运而生.纸作为一种新的检测载体,既能满足传统检测方法的基本要求,还具备了便捷、成本低、检测时间短、适合户外及发展中国家实验室需求等优点.近年来纸基检测发展迅速,在酶联免疫吸附测定法、微流控检测、电传感器、荧光检测、核酸检测等方法中应用广泛.分析并梳理纸基检测方法在以上领域存在的不足,展望其在蛋白质检测、生物标志物筛查和临床诊断中的应用前景,对后续研究具有重要意义.

With the development of modern detection technology which is simple,fast and low cost,various detection methods based on paper came into being.As a new testing carrier,paper not only can meet the basic requirements of traditional testing methods,but also has the advantages of convenience,low cost,short testing time,suitable for outdoor and developing countries'laboratory needs.So in recent years,it has developed rapidly in various testing fields.In this paper,the application of paper in enzyme-linked immunosorbent assay,microfluidic detection,electrical sensor,fluorescence detection,nucleic acid detection and other methods are reviewed.The shortcomings of paper-based detection methods are also reviewed.The application prospect of this new idea in protein detection,biomarker screening and clinical diagnosis is also prospected.

作者：

林虹君 张爱红 李高伟 刘志兵 刘晶

Lin Hongjun;Zhang Aihong;Li Gaowei;Liu Zhibing;Liu Jing(Unit 32311 of PLA,Beijing 102112,China;Institute of Chemical Defense of PLA,Beijing 102205,China)

机构地区：

中国人民解放军32311部队 中国人民解放军陆军防化学院

出处：

《betway官方app 学报：自然科学版》 CAS 北大核心 2021年第5期112-118,共7页

Journal of Henan Normal University(Natural Science Edition)

基金：

国家科技重大专项(2017ZX09304026)。

关键词：

纸 传感器 酶联免疫法 微流控 核酸检测

paper sensor ELISA microfluidic nucleic acid detection

分类号：

Q31 [生物学—遗传学]

纸基检测方法研究进展.pdf