Screening and biological characteristics of an Acid Producing Bacillus strain
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摘要：
使用改良MRS培养基从养殖池塘底泥中分离得到一株具有产酸能力的芽孢杆菌,命名为DN-5.通过形态观察,生理生化指标测定,扫描电镜观察,16S rDNA同源序列比对和gyrB基因及系统发育树分析,确定该菌株为副地衣芽孢杆菌(Bacillus paralichiformis).对该菌株发酵液中的代谢产物进行气相-质谱分析,发现DN-5可产生丁酸、丙酸和乙酸等短链脂肪酸.生物学特性研究表明,DN-5对嗜水气单孢菌(Aeromonas hydrophila)、大肠杆菌(Escherichia coli)和金黄色葡萄球菌(Staphylococcus aureus)均有良好的抑制作用,抑菌圈直径分别达17.40 mm、9.77 mm和21.03 mm.同时,对菌株DN-5进行耐热、耐酸和耐胆盐处理,发现DN-5具有较强的耐热性能和良好的耐酸、耐胆盐能力.对庆大霉素、链霉素、氨苄青霉素等抗生素均表现为敏感(S).DN-5既具有乳酸菌产酸的特性,又具有芽孢杆菌的优势,且具有很好的安全性,在水产养殖中具有广阔的应用前景.
A strain of bacillus capable of acid-producing,named DN-5,was isolated from the pond sediment using MRS medium.Combining microscopic morphology observation,physiological and biochemical index determination,scanning electron microscopic observation,homologous sequence comparison of 16S rDNA gene,gyrB gene and phylogenetic tree analysis,the strain was identified Bacillus paralichiformis.The metabolites of the strain of DN-5 were analyzed by the GC-MS and the assay showed that the strain can produce short chain fatty acids such as butyric acid,propionic acid and acetic acid.The study of biological characteristics showed that DN-5 was resistant to Aeromonas hydrophila,Escherichia coli and Staphylococcus aureus have inhibitory effects,and its inhibition zone diameters are 17.4 mm,9.77 mm and 21.03 mm,respectively.At the same time,the strain DN-5 was treated with heat,acid and bile salt resistance and showed well performance;Moreover,it was sensitive to gentamicin,streptavidin and ampicillin and other antibiotics,so it has good safety and can be used as probiotics.DN-5 not only has the characteristics of lactic acid bacteria producing acid,but also has the advantage of Bacillus sp.,and has broad application prospects in aquaculture.
作者：
张建新 李梦 郭祥瑞 黄梦媛 陈永艳 冯军厂 常绪路
Zhang Jianxin;Li Meng;Guo Xiangrui;Huang Mengyuan;Chen Yongyan;Feng Junchang;Chang Xulu(College of Fisheries,Henan Normal University,Xinxiang 453007,China)
机构地区：
betway官方app 水产学院
出处：
《betway官方app 学报：自然科学版》 CAS 北大核心 2021年第5期96-103,共8页
Journal of Henan Normal University(Natural Science Edition)
基金：
河南省重点科技攻关(212102110106,202102110106,182102110011) 河南省自然科学基金(182300410098).
关键词：
产酸 副地衣芽孢杆菌 菌株筛选 抗逆性
acid-producing Bacillus paralichiformis bacteria screening stress resistance
分类号：
S96 [农业科学—水产养殖]
