Investigation of bacterial resistance in wastewater from a small-scale swine feedlot in Henan province

摘要:

由于我国畜牧养殖业中抗生素长期使用,导致了养殖废水中的抗生素耐药菌(antibioticresistant bacteria,ARB)的大量出现以及抗生素抗性基因(antibioticresistantgene,ARG)的污染,给周边水源以及生态环境带来了巨大的风险.为调查河南区域小型养猪场废水中细菌的耐药性和抗生素抗性基因存在情况,对某小型猪场8个取样点的污水及底泥样品进行检测.利用抗生素药敏试验调查了废水中细菌的耐药性及PCR扩增技术检测抗生素抗性基因的存在情况.结果表明:(1)母猪舍、仔猪舍、育肥舍、妊娠舍和产房5个采样点的废水中细菌的耐药率较高,在经过处理后污水池和沉淀池废水中细菌耐药率显著下降,但与自然环境相比依然较高;(2)四环素类、磺胺类及氨基糖苷类抗生素抗性基因在猪场和生活区细菌中均有较高的检出率,可能在环境中发生迁移,进入附近生活水源区.该研究的调查数据为规范小型养猪场中抗生素的合理使用和进一步展开小规模养猪场中抗生素抗性细菌和抗性基因的污染研究提供了参考.

The excessive use of antibiotics in animal husbandry in China induces the increasing emergence of antibiotic resistance bacteria(ARB)and antibiotic resistance genes(ARG),which results in the wide dissemination of bacterial resistance and great risks to the surrounding water source and the ecological environment.Samples were collected from a small-scale swine feedlot in Henan province to investigate the bacterial resistance and ARGs in the wastewater.Using antimicrobial susceptibility test and PCR amplification technique can demonstrate that:(1)the drug resistance rate of bacteria from the five lagoons including sow houses,nursery houses,finisher houses,gestation houses and farrowing houses are higher than that in the lagoon wastewater,settling tank and living quarters.And there were significant differences in drug resistance;(2)the frequency of detection for ARGs of tetracycline,sulfonamide and aminoglycoside were higher in swine feedlot and living quarters.ARGs may present translocation in environment and transfer into bacteria of water source in living quarters.This study provides valuable data for regulating the fair use of antibiotics and conducting further researches on investigating the ARB and pollution status of ARGs in swine feedlot wastewater.

作者:

秦会玲 常导 刘阳坤 章伟成 付玲 李娜 姚伦广

Qin Huiling;Chang Dao;Liu Yangkun;Zhang Weicheng;Fu Ling;Li Na;Yao Lunguang(Collaborative Innovation Center of Water Security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province,Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North Diversion Project,Nanyang Normal University,Nanyang 473061,China)

机构地区:

南阳师范学院南水北调中线水源区水安全河南省协同创新中心

出处:

《betway官方app 学报：自然科学版》 CAS 北大核心 2021年第1期80-86,共7页

基金:

国家自然科学基金(U1804109) 河南省高等学校重点科研项目(18A230011) 河南省科技厅科技攻关项目(182102310077) 河南省科研服务平台专项(2016151) 南阳师范学院校级科研项目(2018ZX011) 河南省南水北调中线水源区水生态安全创新型科技团队专项(17454).

关键词:

养猪废水 抗生素 耐药性 抗生素抗性基因 抗生素耐药细菌

lagoon wastewater antibiotic resistance antibiotic resistance gene antibiotic resistant bacteria

分类号:

S181 [农业科学—农业基础科学]

河南省某小型猪场废水中细菌耐药性调查.pdf