Analysis of the airborne microbial communities in hospitals and campus under winter severe haze in Xinxiang

摘要：

2017年12月至2018年1月,采集冬季严重雾霾天气下校园室外环境和医院门诊大厅的大气颗粒物样本,通过微生物培养和分子生物学方法研究了气传细菌和真菌的群落组成.结果显示,雾霾发生时校园室外环境中空气微生物的浓度高于医院室内环境,医院的气传细菌多样性明显高于校园,气传真菌多样性则是校园高于医院.值得注意的是,不论是培养法还是高通量测序,都检测出丰度较高的条件致病菌和致敏真菌,例如在医院采集到的黏质沙雷菌(Serratia marcescens)和测序到的丰度较高的隐球菌属(Cryptococcus)等条件致病菌,在校园和医院相对丰度均较高的曲霉(Aspergillus)、青霉(Penicillium)、链格孢霉(Alternaria)、镰刀霉(Fusarium)和木霉(Trichoderma)等致敏真菌.本研究可以为学校学生和医院患者在严重雾霾天气下感染、过敏等疾病的临床诊断、预防及治疗提供理论依据.

In this study,atmospheric particulates were collected from campus outdoor environments and hospital outpatient service hall during severe haze weather from December 2017 to January 2018,and the community composition of airborne microorganisms in the atmospheric particulates was investigated by microbial culture and molecular biology methods.The results showed that the average concentrations of airborne microorganisms in campus outdoor environments were higher than those in hospital outpatient service halls.Besides,the diversity of airborne bacteria in the hospital was higher than that in the campus,while the diversity of airborne fungi was higher in the campus than that in the hospital.It’s worth noting that high abundance of opportunistic pathogenic microorganisms and allergic fungi were detected,such as Serratia marcescens,Cryptococcus in hospital and some allergic fungi,Aspergillus,Penicillium,Alternaria,Fusarium,and Trichoderma with high relative abundance in both campus and hospital.The results of this study can provide theoretical basis for the clinical diagnosis,prevention and treatment of school students and hospital patients with infections,allergies and other diseases in severe haze weather.

作者：

杨清香 辛媛 李慧君 王瑞民

Yang Qingxiang;Xin Yuan;Li Huijun;Wang Ruimin(College of Life Science,Henan Normal University,Xinxiang 453007,China;Henan International Joint Laboratory of Agricultural Microbial Ecology and Technology,Henan Normal University,Xinxiang 453007,China;School of Public Health,Xinxiang Medical University,Xinxiang 453003,China)

机构地区：

betway官方app 生命科学学院 betway官方app 河南省农业微生物生态与技术国际联合实验室 新乡医学院公共卫生学院

出处：

《betway官方app 学报：自然科学版》 CAS 北大核心 2020年第4期78-88,F0002,共12页

基金：

国家基金委-河南省联合基金重点项目(U1904205)。

关键词：

雾霾 大气颗粒物 微生物群落 条件致病菌 致敏真菌

haze atmospheric particulates microbial community opportunistic pathogenic microorganisms allergic fungi

分类号：

Q938.1 [生物学—微生物学]

新乡市冬季严重雾霾下医院和校园气传微生物群落组成研究.pdf