Expression of Cyclin D1,Galectin-3 and ki-67 in differentiated thyroid cancer and its relation with invasion

摘要:

[目的]检测分化型甲状腺癌患者中Cyclin D1,Galectin-3和ki-67表达,探讨其在分化型甲状腺癌局部侵袭中的临床意义.[方法]收集40例侵袭性分化型甲状腺癌(侵袭组)、30例微小乳头状癌(非侵袭组)和20例结节性甲状腺肿(对照组)患者病例,利用免疫组化技术检测Cyclin D1,Galectin-3和ki-67在甲状腺病理切片中的表达.[结果]在侵袭组、非侵袭组和对照组中Cyclin D1表达的阳性率分别为:100%(40/40),53.3%(16/30)和15%(3/20):Galectin-3表达的阳性率分别为:37.5%(15/40),100%(30/30)和0%(0/20):在侵袭组中ki-67增殖指数大于1%病例占侵袭组总数的50%,在非侵袭组中ki-67增殖指数大于1%病例仅占非侵袭组总数的13.3%.同非侵袭组相比较,侵袭组的Cyclin D阳性率明显增高(P<0.01),Galectin-3阳性率明显减低(P<0.01),ki-67阳性率升高(P<0.01),差异具有统计学意义.[结论]Cyclin D1,Galectin-3和ki-67在侵袭性和非侵袭性分化型甲状腺癌中的表达差异有统计学意义,Cyclin D1和ki-67高表达和Galectin-3低表达是分化型甲状腺癌局部侵袭的分子生物学特征之一.

[Objective]To investigate the expression of Cyclin D1,Galectin-3 and ki-67 in differentiated thyroid cancer and its relation with invasion.[Methods]40 patients of invasive well-differentiated thyroid cancer(invasion group), 30 patients of papillary thyroid micro-carcinoma(no-invasion group)and 20 patients of nodular goiter(control group)were recruited and studied.The expression of Cyclin D1,Galectin-3 and ki-67 were detected and analysed with immunohistochemistry methods.[Results]The result of immunohistochemistry staining displayed that the positive rate of Cyclin D1 were 100%(40/40),53.3%(16/30)and 15%(3/20)in invasion group,no-invasion group and control group.The positive rate of Galectin-3 were 37.5%(15/40),100%(30/30)and 0%(0/20)in invasion group,no-invasion group and control group.Ki-67 index of 50%(20/40)patients in invasion group were more than 1%,only 13.3%(4/30)patients in no-invasion group were more than 1%.It was confirmed with immunohistochemistry methods that Cyclin D1 and ki-67 positive rate of invasion group was obviously higher than that of no-invasion group and control group(P<0.01),Galectin-3 positive rate of invasion group was obviously lower than that of no-invasion group(P<0.01).[Conclusion]There are significantly differences in expressions of between invasive and none-invasive differentiated thyroid cancer.High expression of Cyclin D1 and ki-67,low expression of Galectin-3 were risk factors influencing extra-thyroidal invasion of differentiated thyroid cancer.

作者:

何时知 房居高 陈佳铭 刘擘 庄欢 沈茜茜 廉猛 马泓智 侯丽珍 张雪 何小金 钟琦

He Shizhi;Fang Jugao;Chen Jiaming;Liu Bo;Zhuang Huan;Shen Xixi;Lian Meng;Ma Hongzhi;Hou Lizhen;Zhang Xue;He Xiaojin;Zhong Qi(Department of Otolaryngology Head Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;Department of Pathology,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;Department of Thyroid Surgery,The First Hospital of Hebei Medical University,Shijiazhuang 050031,China)

机构地区:

首都医科大学附属北京同仁医院耳鼻咽喉头颈外科 首都医科大学附属北京同仁医院病理科 河北医科大学第一医院甲状腺外科

出处:

《betway官方app 学报：自然科学版》 CAS 北大核心 2020年第2期113-117,共5页

基金:

首都卫生发展专项项目(2018-2-2054) 首都卫生发展科研专项项目(2018-1-2052)联合资助,北京市医院管理局“登峰”人才培养计划(DFL20180202).

关键词:

分化型甲状腺癌 侵袭 CYCLIN D1 GALECTIN-3 KI-67

differentiated thyroid carcinoma invasion Cyclin D1 Galectin-3 ki-67

分类号:

R736.1 [医药卫生—肿瘤]

分化型甲状腺癌中Cyclin D1,Galectin-3和ki-67的表达及其与侵袭的关系.pdf