Effects of exercise training on repair of rat cartilage injury by electrospinning nanofiber loaded with mesenchymal stem cells

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[目的]研究有氧运动训练在荷载有脂肪充质干细胞(ADSCs)的纳米静电纺丝材料修复大鼠关节软骨损伤中的作用与影响.[方法]分离培养大鼠ADSCs,体外扩增,流式鉴定,荷载于聚碳酸亚丙酯/聚己内酯/左旋聚乳酸-磷酸四钙(PPC/PCL/g-TTCP)纳米静电纺丝载体上,植入到大鼠膝关节软骨缺损处.实验大鼠随机分为2组:1)正常对照组;2)运动训练组.训练组采用中等强度的水平跑台运动(12 m/min的速度,持续30 min).术后第3周开始训练,每周训练5 d,每天训练2次,训练8周后,对修复组织进行苏木素-伊红(HE)和Ⅱ型胶原等染色和组织学检查,采用Wakitani评分系统进行软骨修复评分,采用Graphpad Prism 6.01进行统计分析.[结果]PPC/PCL/g-TTCP与大鼠ADSCs具有很好的相容性,可以成功荷载和诱导成骨分化;在运动训练组的关节软骨修复评分指标中,细胞形态,基质染色强度,表面规整度,修复软骨相对厚度以及移植物与宿主整合度5项指标评分均显著优于对照组;Ⅱ型胶原和HE染色显示运动训练组软骨细胞增生明显,Ⅱ型胶原阳性细胞占比显著提高.[结论]中强度运动训练可以显著提高荷载ADSCs的纳米静电纺丝对大鼠关节软骨损伤的修复水平.

[Aims]To study the functions and effects of aerobic training in the repair of rat articular cartilage injury by electrospinning nanofibers loaded with adipose-derived mesenchymal stem cells(ADSCs).[Methods]Rat ADSCs were isolated,cultured and expanded in vitro.ADSCs were examined by flow cytometry.ADSCs were loaded on polypropylene carbonate/poly ε-caprolactone/poly L-lactic acid-grafted tetracalcium phosphate(PPC/PCL/g-TTCP)nano electrospinning carriers and implanted into the cartilage defects of rat knee joints.Experimental rats were randomly divided into two groups:1)Normal control group;2)Exercise training group.The training group uses moderate-intensity horizontal treadmill exercise(12 meters per minute,last for 30 minutes).Training began in the third week after surgery.Training was conducted twice daily and five days a week.After eight weeks of training,hematoxylin-eosin(HE)and typeⅡcollagen were stained and histologically examined.Wakitani score system was used to evaluate the cartilage repair degree.Statistical analysis was performed by using Graphpad Prism 6.01.[Results]Rat ADSCs can proliferate and differentiate into osteocytes on PPC/PCL/g-TTCP;In the exercise training group,the scores of five indexes(cell morphology,matrix staining intensity,surface regularity,cartilage thickness and graft-host integration)were significantly lower than that of the control group;HE and typeⅡcollagen staining showed that the proliferation of chondrocytes and the proportion of typeⅡcollagen was significantly increased in the training group.[Conclusion]Moderate-intensity exercise training can significantly improve the level of repair of rat articular cartilage injury by electrospinning nanofiber loaded with ADSCs.

作者:

仝敬平

Tong Jingping(College of Physical Education,Henan Normal University,Xinxiang 453007,China)

机构地区:

betway官方app 体育学院

出处:

《betway官方app 学报：自然科学版》 CAS 北大核心 2020年第1期101-108,共8页

基金:

国家自然科学基金(81803075)。

关键词：

有氧运动软骨损伤软骨修复间充质干细胞静电纺丝

aerobic exercise cartilage injury cartilage repair mesenchymal stem cell electrospinning nanofiber