Identification and phylogenetic analysis of powdery mildew on Polygonum aviculare in Zhoukou,Henan Province
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摘要：
通过形态观察、分子鉴定、科赫法则验证和系统进化分析对河南周口地区萹蓄所感病原菌进行鉴定.结果表明:该病原菌菌丝体外生,菌丝有隔膜.分生孢子梗圆柱形,包含3个细胞;分生孢子串生,近柱形,大小为(22.0~35.5)μm×(10.5~16.5)μm.闭囊壳近卵形,含有3~5个子囊孢子.科赫法则检测发现,病原菌回接发病与自然发病症状一致.分子生物学和系统进化分析结果显示,该病原菌ITS序列(KX757832.1)与已报道的蜡叶峨参独活白粉菌(KF111807.1)、洋常春藤独活白粉菌(KP055630.1)、胡萝卜独活白粉菌(KC480605.1)和酸模独活白粉菌(KY073878.1)序列聚为一枝,其同源性分别为98.40%、98.57%、98.40%和98.57%.初步推测,河南周口地区萹蓄所感白粉病原菌为独活白粉菌.
In this study, the morphological observation, molecular identification, Koch’s postulates verification and phylogenetic analysis were used to identify the pathogen on Polygonum aviculare in Zhoukou, Henan province. The results showed that the mycelia were exogenously and the hypha were separate. The conidiophore was cylindrical, which contains three cells. The string of conidia were near column, scale was (22.0-35.5)μm ×(10.5-16.5)μm. The cleistothecium was nearly ovoid and contains 3-5 ascospores. The symptoms of inoculation pathogen was same to the nature, which was identified through Koch’s postulates. The internal transcribed spacer (ITS)(KX757832.1) sequence is clustered with Erysiphe heraclei on Anthriscus cerefolium (KF111807.1), Hedera helix (KP055630.1), Daucus carota (KC480605.1) and Rumex acetosa (KY073878.1) in one clade, the sequence similarity were 98.40%, 98.57%, 98.40% and 98.57% respectively. We presume that the pathogen infected on P. aviculare was E. heraclei in Zhoukou.
作者：
王伟 徐克东 于德水 李成伟 吴建宇
Wang Wei;Xu Kedong;Yu Deshui;Li Chengwei;Wu Jianyu(College of Life Science,Henan Agricultural University,Zhengzhou 450002,China;Key Laboratory of Plant Genetics and Molecular Breeding,Zhoukou Normal University,Zhoukou 466001,China;Henan Institute of Science and Technology,Xinxiang 453003,China)
机构地区：
河南农业大学生命科学学院 周口师范学院植物遗传与分子育种重点实验室 河南科技学院
出处：
《betway官方app 学报：自然科学版》 CAS 北大核心 2019年第4期105-110,共6页
基金：
国家自然科学基金面上项目(31872129) 国家自然科学基金委员会与国际农业研究磋商组织合作研究项目(3171101241) 河南自然科学基金项目(162300410107)
关键词：
萹蓄 白粉病菌 科赫法则 独活白粉菌
Polygonum aviculare powdery mildew Koch’s postulates Erysiphe heraclei
分类号：
S436.43 [农业科学—农业昆虫与害虫防治]
