Effects of copper on growth and expression of immune-related genes in kidney of grass carp (Ctenopharyngodon idellus)
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摘要：
为了探讨水体重金属铜对草鱼(Ctenopharyngodon idellus)的生长和免疫的毒性作用,分别将草鱼在不同质量浓度的Cu^2+溶液中暴露30、60和90d,检测了草鱼的均重和增重率的变化,以及草鱼肾脏中6种免疫相关基因表达的变化.结果表明:与对照组相比,各质量浓度Cu^2+暴露均使草鱼在30、60和90d后的均体质和增体质率下降;0.40和0.60mg·L^-1质量浓度Cu^2+暴露60和90d后,IL-1β,CCL4和TNF-α基因在草鱼肾脏中的表达量均极显著升高(P<0.01),而IFN-γ和IgM基因在草鱼肾脏中的表达量均显著或极显著下降(P<0.05或P<0.01);Cu^2+暴露30、60和90d后,草鱼肾脏中MT基因表达量均随着Cu^2+质量浓度的增加先升高后下降,在Cu^2+质量浓度为0.10mg/L时达到最大值.综上,高质量浓度和长时间的Cu^2+暴露阻碍了草鱼的生长,引起了草鱼肾脏细胞的炎症反应,降低了草鱼的免疫功能.
To investigate the toxic effect of waterborne copper on the growth and immunity of grass carp ( Ctenopharyngodon idellus ), grass carps were exposed in different concentrations of Cu^2+ solutions for 30, 60 and 90 days, then the weight and weight gain rate, the expression changes of six immunity-related genes in kidney of grass carps were measured. The results showed that: Compared with the control group, the average weights and weight gain rates all decreased under different concentrations of Cu^2+ exposures for 30, 60, and 90 days;After exposure to 0.40 and 0.60 mg·L^-1 Cu^2+ for 60 and 90 days, the expression levels of IL-1β, CCL4 , and TNF-α genes in kidney of grass carp were extremely significantly increased ( P <0.01), however, the expression levels of IFN-γ and IgM genes were significantly or extremely significantly decreased ( P <0.05 or P <0.01);After 30, 60 and 90 days of Cu^2+ exposures, the MT gene expression in kidney of grass carp increased at first and then decreased with the increasing of Cu^2+ concentration, and reached the maximum value at the Cu^2+ concentration of 0.10 mg·L^-1 . In conclusion,high concentration and long time Cu^2+ exposure hindered the growth of grass carp, caused inflammatory reaction of kidney cells, and weakened the immune function of grass carp.
作者：
江红霞 叶凯甲 凌洁彬 王李博 孔祥会 李学军
Jiang Hongxia;Ye Kaijia;Ling Jiebin;Wang Libo;Kong Xianghui;Li Xuejun(College of Fisheries,Henan Normal University,Xinxiang 453007,China;Dengzhou Fisheries Management Station,Dengzhou 474150,China)
机构地区：
betway官方app 水产学院 邓州市水产管理站
出处：
《betway官方app 学报：自然科学版》 CAS 北大核心 2019年第4期84-91,共8页
基金：
河南省科技厅重点科技攻关项目(192102110081) 河南省教育厅科学技术重点研究项目(13A240509) betway官方app 个人科研项目结余经费资助专项(20180531) betway官方app 博士科研启动经费项目(qd17142)
关键词：
草鱼 铜 生长 免疫相关基因 基因表达
Ctenopharyngodon idellus copper growth immune-related gene gene expression
分类号：
Q786 [生物学—分子生物学]
