Expression of CDK16 in esophageal squamous cell carcinoma and its effect for the tumorigenesis and development of tumor
- 分享到:
摘要：
目的研究发现,细胞周期素依赖性激酶(cyclin dependent kinases,CDKs)家族成员与食管鳞癌的发病密切相关,但细胞周期素依赖性激酶16(CDK16)对食管鳞癌发病的影响尚不清楚.本研究旨在探讨CDK16在人食管鳞癌组织中的表达及生物学意义.方法应用组织芯片技术结合免疫组织化学技术检测45例食管鳞癌组织、45例癌旁组织中CDK16蛋白的表达情况,并使用统计分析软件研究CDK16蛋白的表达与食管鳞癌临床病理特征之间的关系.结果 CDK16在食管鳞癌组织中呈阳性表达,且表达强度与肿瘤分化程度呈负相关.另外,CDK16在食管鳞癌细胞中的表达位置与肿瘤的分化程度紧密相关.CDK16的表达与食管鳞癌的分化程度、淋巴结转移及TNM临床分期均具有显著的相关性(P<0.05),而与患者的年龄、性别以及肿瘤的部位、最大径、病理形态没有显著的相关性(P>0.05).结论 CDK16在食管鳞癌中的高表达与食管鳞癌的发生发展及转移密切相关.检测CDK16的表达有助于食管鳞癌的临床诊断和预后评估.
Aims Studies found that members of cyclin dependent kinase(CDKs)family were closely related to esophageal squamous cell carcinoma,but cyclin dependent kinase 16(CDK16)on incidence of esophageal squamous cell carcinoma was not clear.The aim of this study is to investigate the expression and biological significance of CDK16 in esophageal squamous cell carcinoma.Methods The expression of CDK16 was detected in tumor tissue microarrays of 45 cases of esophageal squamous cell carcinoma tissues,45 cases of paraneoplastic tissues by immunohistochemical method.The relationship between the expression of CDK16 and the clinicopathological features of esophageal squamous cell carcinoma was analysed by using mathematical statistical analysis software.Results The expression of CDK16 was positive in esophageal squamous cell carcinoma,and the expression intensity was negatively correlated with the degree of tumor differentiation.In addition,the location of CDK16 in esophageal squamous cell carcinoma cells is closely related to the degree of tumor differentiation.The expression of CDK16 was significantly correlated with the degree of tumor differentiation,lymph node metastasis and TNM clinical stage of ESCC(P<0.05),but not with age,sex,tumor location,maximum diameter and pathological morphology(P>0.05).Conclusion The high expression of CDK16 in esophageal squamous cell carcinoma is closely related to the occurrence and development of esophageal squamous cell carcinoma.Detection of CDK16 expression is helpful to the clinical diagnosis and prognosis assessment of esophageal squamous cell carcinoma.
作者：
郑皓 景嘉楠 董慧 李铁鹏 夏鑫 郭燕洁 董子明
Zheng Hao;Jing Jianan;Dong Hui;Li Tiepeng;Xia Xin;Guo Yanjie;Dong Ziming(School of Basic Medical Sciences Zhengzhou University,Zhengzhou,450001;College of Public Health Zhengzhou University,Zhengzhou,450001;Department of Clinical Medicine,Zhengzhou University,Zhengzhou,450001;Henan Cancer Hospital,Zhengzhou 450008,China)
机构地区：
郑州大学基础医学院 河南省肿瘤医院 郑州大学公共卫生学院 郑州大学临床医学系
出处：
《betway官方app 学报：自然科学版》 CAS 北大核心 2019年第1期118-124,共7页
基金：
河南省高等学校重点科研项目(16A310004) 郑州大学青年教师启动基金(1511328002) 郑州大学国家级大学生创新创业训练计划项目(201510459071 201610459079)
关键词：
细胞周期素依赖性激酶16 组织芯片 免疫组织化学
esophageal squamous cell carcinoma cyclin dependent kinase 16 tissue microarray immunohistochemistry
分类号：
R363 [医药卫生—病理学]
