Embryonic development of Megalobrama amblycephala and optimization on the observation method
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摘要：
通过人工繁殖获得团头鲂受精卵,在体视显微镜下对其胚胎和早期仔鱼发育过程进行了连续观察、拍照与测量.结果显示,团头鲂受精卵呈圆球形,具黏性,卵径(1.18±0.09)mm;在水温(21±1)℃条件下,团头鲂胚胎发育从受精到孵化历时39h10min,经历了胚盘形成、卵裂期、囊胚期、原肠胚期、神经胚期、器官形成期和出膜期7个发育阶段;初孵仔鱼体长(3.44±0.29)mm,出膜后第4d仔鱼鳔完全充气,卵黄囊消失;观察时,受精卵至尾鳍出现期胚胎置0.7%生理盐水中,肌肉效应期和后面各时期的胚胎经4%多聚甲醛固定后置3%甲基纤维素中拍照可获得良好效果.该工作为研究团头鲂胚胎早期发育过程提供了实时和动态的图像资料,并为鱼类胚胎发育观察提供了基本技术参考.
This study investigated the development process of embryos and early larval fish from Megalobrama amblycephala under stereoscopic microscope.The fertilized eggs were sticky and round with a diameter at(1.18±0.09)mm.The embryonic development underwent seven stages as blastoderm formation,cleavage,blastula,gastrula,neurula,organ formation and hatching stage.Under incubation temperature of(21±1)℃,the embryos take39h10min to hatch into larvae,which is near(3.44±0.29)mm in length.The swim bladder of the larvae was inflated completely and the yolk sac disappeared in the fourth day.An optimized protocol was obtained to achieve the better observation:Embryos before muscle functioning stage were treated with0.7%saline,or embryos after muscle functioning stage were fixed in4%PFA and immersed into methylellulose.Taken together,the present study provided dynamic images on the embryonic development of M.amblycephala and supplied a basic technical skill on observation of fish embryos.
作者：
于淼 方健 薛亭 陈凯 李玲玉 潘启华 王艺舟 邓羽 陈天圣
Yu Miao;Fang Jian;Xue Ting;Chen Kai;Li Lingyu;Pan Qihua;Wang Yizhou;Deng Yu;Chen Tiansheng(College of Fishery, Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation,Henan Normal University, Xinxiang 453007, China;College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China)
机构地区：
betway官方app 水产学院 华中农业大学水产学院
出处：
《betway官方app 学报：自然科学版》 CAS 北大核心 2018年第1期89-94,共6页
基金：
国家重点基础研究计划(2013CB967700) 华中农业大学自主科技创新基金(2013RC014) 河南省科技厅科技计划项目(102300410104) betway官方app 青年科学基金(2013qk15) 河南省水产养殖省级重点学科资助项目.
关键词：
团头鲂 胚胎发育 鲤科 显微观察 甲基纤维素
blunt snout bream (Megalobrama amblycephala) embryonic development Cyprinidae microscope observation methylcellulose
分类号：
S917.4 [农业科学—水产科学]
