Quantitative Distribution and Functional Analysis of LTR Retrotransposons in Gossypium hirsutum

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摘要:

LTR(Long terminal repeat)反转录转座子是真核生物基因组中普遍存在的一类遗传因子,它们以RNA为媒介在基因组中不断自我复制.在高等植物中,LTR反转录转座子是基因组的重要成分之一.本研究通过多种方法挖掘并注释了陆地棉基因组中的LTR反转录转座子,结果表明陆地棉基因组LTR反转录转座子的Gypsy超家族与基因的分布呈近似的反比关系,而Copia超家族在各染色体的起始端有较多的分布.通过皮尔森相关系数发现陆地棉LTR反转录转座子的拷贝数与染色体大小之间有强相关性.在LTR反转录转座子上游和下游分布的基因具有类似的富集特征,其分子功能主要集中在结合和催化活性等方面.本研究结果加深了对陆地棉LTR反转录转座子的认识,为深入研究棉花基因组提供了重要数据支撑.

LTR （Long terminal repeat） retrotransposons in eukaryotes are mobile genetic elements with ubiquitous distri-bution, these elements can amplify themselves via RNA intermediates and increase their copy numbers in genome. In high plants, LTR retrotransposon is an important component of genome. In this study, LTR retrotransposons of Gossypium hirsu- turn were excavated and annotated by a variety of methods,the results showed that the Gypsy superfamily of LTR retrotrans-posons in Gossypium hirsutum was approximately inversely related to the distribution of genes, while Copia superfamily dis-tributes more at the starting terminal of each chromosome. Pearson correlation coefficients showed that there was a strong cor-relation between the LTR retrotransposons copy number and chromosome size in Gossypium hirsutum. Furthermore〉 the genes enrichment in either upstream or downstream of the LTR retrotransposons exhibit similar characteristics, and the molecular functions mainly focus on binding and catalytic activity etc. Our research will lead to a better understanding of LTR retrotrans-posons in Gossypium hirsutum genome and provide important data support for further study of cotton genome.

作者:

刘震卢全伟张国强彭仁海

机构地区:

安阳工学院生物与食品工程学院中国农业科学院棉花研究所

出处:

《betway官方app 学报：自然科学版》 CAS 北大核心 2017年第6期72-78,共7页

基金:

国家自然科学基金(31471548) 棉花生物学国家重点实验室开放课题(CB2015A21) 河南省高等学校重点科研项目(18A180010) 安阳工学院校科研基金(YJJ2017007)

关键词:

陆地棉 LTR反转录转座子分布功能

Gossypium hirsutum LTR Retrotransposons distribution function

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