Optimizing Extraction Technology of Anthocyanin from Purple Chrysanthemum Petals Based on Response Surface Methodology
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摘要:
采用响应面分析法优化紫色菊花花瓣中花青素苷的提取工艺.本试验以紫色菊花品种红五九自然晾干陈放后花瓣为材料,采用Box-Behnken中心组合试验设计,获得多元二次回归方程,并预测紫色菊花花瓣花青素苷得率.结果表明,分别以酸性乙醇和酸性甲醇为浸提液时,紫色菊花花瓣花青素苷的最佳提取工艺参数为:乙醇浓度90%(V/V),盐酸浓度0.4mol·L-1、盐酸与乙醇体积比1∶1、温度40℃、料液比1∶70;甲醇浓度100%(V/V),盐酸浓度0.1mol·L-1、盐酸与甲醇体积比1∶1、温度42℃和料液比1∶70,一次浸提的花青素苷得率分别为8 266.03μg·g-1和7 916.04μg·g-1,与前期研究通过正交试验获得的最优工艺参数下的花青素苷得率均有明显提高,以酸性乙醇为浸提液效果较好,可为紫色菊花花瓣花青素苷的生产提供参考.
Based on response surface methodology. In this study,the optimum extraction technological conditions of an-thocyanin from purple petals of chrysanthemum was selected The dry purple petals of chrysanthemum cultivars ＂ Hongwujiu＂ which had been laid for a period of time were used as materials. By Box-Behnken design,a quadric regression equation for pre-dicting the yield of anthocyanin were established.The results showed that the ethanol concentration was 90%,the optimal pa-rameters were 0.4 mol·L-1 for the hydrochloric acid concentration, 1 ： 1 for the volume ratio of ethanol to hydrochloric acid, 40℃ for the extraction temperature, liquid to material ratio of 1 ： 70. And the methanol concentration was 100% , the optimal parameters were 0.1 mol·L-1 for the hydrochloric acid concentration 1：1 for the volume ratio of methanol to hydrochloric acid,42 °Cfor the extraction temperature,liquid to material ratio of 1：70. Under the extracting system with acid ethanol and acid methanol,the yield of anthocyanin were 8 266.03 pg·g-1 and 7 916.04 pg· g-1, respectively, so the former was higher than the latter,and they were higher than the yield of anthocyanin obtained under the extraction conditions selected by orthogo-nal experimental design,which could be useful for the mass production of anthocyanin from the purple chrysanthemum.
作者:
刘海英 邢晨涛 李凤阳 王凤杰 王亚涛 刘宁
机构地区:
betway官方app 生命科学学院 betway官方app 新联学院
出处:
《betway官方app 学报：自然科学版》 CAS 北大核心 2017年第6期58-64,共7页
基金:
基金项目:河南省科技厅工业类重点攻关(142102210057)
关键词:
菊花 花青素苷 提取 最优工艺条件 响应面法
Chrysanthemum anthocyanin extraction optimum technological condition response surface method
分类号:
R917 [医药卫生—药物分析学]
