Effect of Chlorogenic Acid on LPS-induced COX-2 in Vivo and Vitro

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摘要:

探讨绿原酸对脂多糖致炎症小鼠体内体外COX-2蛋白表达的影响.脂多糖滴鼻造模建立小鼠急性肺损伤模型,脂多糖诱导小鼠巨噬细胞RAW264.7建立细胞炎症反应模型,应用Weston blot法,检测小鼠肺组织内以及RAW264.7巨噬细胞中COX-2的蛋白表达.绿原酸在小鼠体内体外均能有效降低脂多糖诱导的COX-2的蛋白表达,并能促进COX-2蛋白的降解.降低脂多糖诱导的COX-2的蛋白水平是绿原酸抗炎的机制之一.

The object of this study was to explore the effect of chlorogenic acid on LPS-induced COX-2 in vivo and vitro.The mice induced by LPS was used as an acute lung injury model.The RAW264.7 cells induced by LPS was used as an cell inflammatory model.The expression of COX-2in lung tissue as well as in RAW264.7 cells was detected by Western blot.The results showed that chlorogenic acid could inhibit the expression of COX-2 protein in vivo and vitro effectively and promote the protein degradation of COX-2 protein in RAW264.7 cells.Reducing the protein level of LPS-induced COX-2 is one of the mechanisms for the anti-inflammatory action of chlorogenic acid.

作者:

抗晶晶张丽娟刘晓宁殷志敏

机构地区:

黄河科技学院医学院南京师范大学生命科学学院

出处:

《betway官方app 学报：自然科学版》 CAS 北大核心 2017年第5期49-52,共4页

基金:

河南省科技厅科技攻关项目(172102310208) 河南省教育厅自然科学计划项目(16A180046)

关键词:

绿原酸急性肺损伤 COX-2

chlorogenic acid acute lung injury COX-2

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