Effect of imidazole derivative KK-42 on mineralization of the cuticle in Macrobrachium nipponense

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摘要:

甲壳动物为了生长必须周期性地褪去旧表皮,新表皮的形成及矿化与蜕皮周期密切相关.前期研究发现,KK-42能够缩短日本沼虾蜕皮周期、加速表皮的形成.为了进一步探究KK-42对表皮矿化的影响,以矿化相对稳定的蜕皮间期(C期)头胸甲表皮为参照,选择蜕皮后3,6,12 h的头胸甲,采用扫描电镜/能谱仪法(SEM/EDX),在超微水平初步研究了表皮的矿化进程及KK-42处理对表皮矿化的影响.结果显示,对照组头胸甲表皮中钙、镁、磷信号在蜕皮后3 h开始出现,6 h钙、磷信号在上/外表皮的边缘处密集分布,此后逐渐向表皮内侧延伸.镁信号一直呈弥散状分布.半定量数据分析显示,与蜕皮后3 h相比,6,12 h及C期表皮中,钙原子比例分别提高了67.86%(P<0.01),402.78%(P<0.01),2191.67%(P<0.01);镁原子比例分别提高了95.92%(P<0.05),122.45%(P<0.05),165.31(P<0.05);磷原子比例分别提高了82.46%(P<0.05),205.26%(P<0.01),591.23(P<0.01).在KK-42处理组,蜕皮后3,6,12 h,表皮中的钙、磷信号分布一直呈离散状,钙原子比例分别比对照组降低了16.67%(P>0.05),45.54%(P<0.05),52.5%(P<0.01),磷原子比例分别比对照组降低了15.79%(P>0.05),45.19%(P<0.05),50%(P<0.01);C期表皮中的钙、磷信号出现密集现象,但信号强度比相应对照组分别低31.88%(P<0.01)和5.08%(P>0.05).KK-42处理后镁信号的分布和强度与对照组均无明显差异.结果表明:日本沼虾表皮的矿化始于蜕皮后3 h,KK-42处理能明显抑制日本沼虾头胸甲表皮的矿化,表皮中钙、磷的沉积显著降低.

Crustaceans have to replace the old cuticle periodically to permit their growth,thus the molt cycle is closely related to the formation and mineralization of the new cuticle.In previous studies we found that KK-42 treatment could shorten molt cycle and accelerate the formation of the cuticle in Macrobrachium nipponense.To explore the possible mechanism of KK-42,carapaces at 3,6 and 12 h were employed to investigate calcification of cuticle and the effect of KK-42 treatment on calcification at ultrastructural level using the observation of paraffin section by scanning electron microscope-energy dispersive spectrometer(SEM-EDS)with reference of carapaces at intermolt(C stage)during which mineralization of the cuticle is relatively stable.The results showed that calcium magnesium and phosphorous signals began to appear in the cuticle at 3 h after ecdysis.Calcium and phosphorous signals became dense and extended from the epicuticle/exocuticle boundary to the inner side of cuticle in control group at 6 h after ecdysis.Magnesium signals were dispersively distributed.Semi-quantitative data analysis showed that,compared with control group of 3 h after ecdysis,at 6,12 h after molting and C stage,atomic percent of calcium increased by 67.86%(P<0.01),402.78%(P<0.01)and 2191.67%(P<0.01)respectively,atomic percent of magnesium increased by 95.92%(P<0.05),122.45%(P<0.05),165.31(P<0.05)respectively,atomic percent of phosphorous improved by 82.46%(P<0.05),205.26%(P<0.01),591.23(P<0.01)respectively.In KK-42 treatment group,the distribution of calcium and phosphorous signals in cuticle was discrete during 3,6,12 h after ecdysis.Compared with the corresponding control group,atomic percent of calcium decreased by 16.67%(P>0.05),45.54%(P<0.05)and 52.5%(P<0.01)respectively,atomic percent of magnesium decreased by 15.79%(P>0.05),45.19%(P<0.05),50%(P<0.01)respectively.Calcium and magnesium signals were dense until C stage,but the signal intensity was 31.88%(P<0.01)and 5.08%(P>0.05)lower than that in control group respectively.No significant difference in the distribution and intensity of magnesium signals was observed between the control and treatment group.The results indicated that mineralization of cuticle in M.nipponense started in 3 h after molt,and KK-42 treatment could significantly inhibit the mineralization of carapace cuticle,for deposition of calcium and phosphorous in the cuticle decreased significantly.

作者:

杜娟张俊芳岳凯迪彭彦新杨洪宁黔冀

Du Juan;Zhang Junfang;Yue Kaidi;Peng Yanxin;Yang Hong;Ning Qianji(College of Life Sciences,Henan Normal University,Xinxiang 453007,China)

机构地区:

betway官方app 生命科学学院

出处:

《betway官方app 学报：自然科学版》 CAS 北大核心 2023年第1期144-148,共5页

Journal of Henan Normal University(Natural Science Edition)

基金:

国家自然科学基金(31873047) 河南省自然科学基金(182300410033).

关键词:

日本沼虾表皮扫描电镜/能谱仪法矿化 KK-42