Effects of Hypoxia on the Growth and Cisplatin Sensitivity in ECa9706 Cells

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摘要：

着重探讨低氧对食管癌ECa9706细胞生长及顺铂敏感性的影响.ECa9706细胞分别培养于顺铂干预的常氧和低氧(1%O2)环境下,倒置显微镜观察细胞形态,MTT法检测细胞活力,流式细胞法检测细胞凋亡和细胞周期.结果显示:与常氧环境下培养相比,低氧下细胞形态变长、变大,细胞自第7d后生长缓慢.常氧下顺铂作用细胞48h的IC50为(1.180±0.056)μM,低氧下为(2.675±0.063)μM.培养48h后,常氧对照组及常氧高浓度顺铂组(1.18μM)阻滞细胞周期于G1期,低氧下各组细胞周期阻滞于S期.低氧导致顺铂诱导的细胞凋亡率较常氧下降低.表明:低氧环境影响了细胞的形态、增殖及细胞周期,低氧下细胞对顺铂的细胞毒作用敏感性降低.

This study focused on the effects on cell growth and cisplatin sensitivity in esophageal cancer ECa9706 cells under hypoxia. ECa9706 cells were cultured under normoxia or hypoxia （1%O2 ） treated by cisplatin respectively, and then, the cell morphology were observed by inverted microscope, cells activation was tested by MTT assays, apoptosis and cell cycle were detected by flow cytometry respectively. Our research revealed that ECa9706 cells cultured in hypoxia were longer, bigger than that of cells in normoxia, and grew slowly since the cells were treated hy cisplatin at 7 day under hypoxia. The IC50 value for ECa9706 cells under normoxia or hypoxia treated with cisplatin for 48 h were （1. 180 ± 0. 056） 9M and （2. 675 ±0. 063） μM respectively. Cells cycle was arrested at G1 phase both the cells were cultured under normoxia and treated by cisplatin at 1.18 #M under normoxia for 48 h respectively . The cells cycle were arrested in S phase under hypoxia during a 48 h period. The rate of apoptosis induced by cisplatin under hypoxia were decreased than that of cells in normoxia. It is concluded that hy- poxia affects the morphology, proliferation and cell cycle, and decreases the effect of cisplatin on cells cytoactivity in ECa9706 cells cultured in hypoxia.

作者：

杨联河赵雪艳牛春玲辛凯陈玉龙

机构地区：

河南中医学院基础医学院河南中医学院第三附属医院

出处：

《betway官方app 学报：自然科学版》 CAS 北大核心 2015年第5期107-111,共5页

基金：

国家自然科学基金面上项目(81373535) 河南省科技攻关计划项目(142102310038) 河南省教育厅科学技术研究重点项目(14A360025) 河南中医学院博士基金项目(BSJJ2011-01)

关键词：

NORMOXIA HYPOXIA ECa9706 CISPLATIN drug-sensitivity

normoxia hypoxia ECa9706 cisplatin drug-sensitivity

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