Rapid Screening of 130 Toxicants in Blood by Direct Analysis in Real Time-orbitrap High-resolution Mass Spectrometry

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摘要：

血液样品经乙酸乙酯提取、离心、取上清液5μL筛网进样,采用正离子模式扫描进行实时直接分析离子源-静电场轨道阱高分辨质谱（DART-Orbitrap MS）分析,通过比对130种毒物精确分子质量数据库,建立了血液中毒物快速筛查分析方法.结果表明：130种毒物的检出限（以3倍S/N计算）为0.2~50μg/L,质量精确度均在5×10-6μg以内.该方法样品前处理简单、溶剂消耗少,具有分析时间短、灵敏度高的特点,适用于血液中毒物的快速筛查分析.

Take out the blood samples supernatant 5μL mesh screen injection modules, which has been extracted by eth- yl acetate and then centrifuged. 130 toxicants were analyzed by direct analysis in real time-orbitrap high-resolution mass spec- trometry （DART-Orhitrap MS） in positive mode. By comparing the 130 toxicants accurate mass database, a high-throughput method for the screening of toxicants in blood was developed. The results showed the detection limits of these 130 toxicants ranged from 0.2 to 50 μg/L and Mass error （〈5 × 10 6μg）. The method is simple, rapid, sensitive with a little less solvent consumptive. It is suitable for screening and confirmation of toxicant in blood samples.

作者：

孙雨安王珂李振兴于文浩王国庆

机构地区：

郑州轻工业学院材料与化学工程学院

出处：

《betway官方app 学报：自然科学版》 CAS 北大核心 2015年第3期69-73,共5页

基金：

国家自然科学基金(21075113 21171150)

关键词：

实时直接分析(DART) 静电场轨道阱高分辨质谱筛查血液毒物

direct analysis in real time （DART） orbitrap high-resolution mass spectrometry screening blood toxicant

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